A novel Gram-negative, mesophilic, slightly halophilic, catalase- and oxidase-positive, obligately aerobic bacterium, designated strain YIM-C238, was isolated from a sediment sample from a salt lake in the Qaidam Basin, north-west China. Cells were non-sporulating, non-motile, straight to slightly curved rods. Coccoid bodies and filaments of varying length developed in older cultures. Growth occurred with 0.5–6 % (w/v) NaCl [optimum, 2–3 % (w/v) NaCl], at pH 6.0–10.0 (optimum, pH 7.0–8.0) and at 10–40 °C (optimum, 25–30 °C). The major cellular fatty acids were anteiso-C, 3-OH iso-C and anteiso-C 10, and menaquinone 6 was the sole respiratory quinone. Non-diffusible carotenoid pigments were produced. Flexirubin pigments were absent. The genomic DNA G+C content was 35.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM-C238 should be assigned to the genus . The sequence similarities between the isolate and the type strains of members of the genus were in the range 95.5–97.0 %. The combination of phylogenetic analysis, DNA–DNA hybridization data, phenotypic characteristics and chemotaxonomic differences supported the view that strain YIM-C238 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM-C238 (=CCTCC AA 207030=KCTC 22166).


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  1. Bernardet, J.-F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  2. Bowman, J. P., McCammon, S. A., Lewis, T., Skerratt, J. H., Brown, J. L., Nichols, D. S. & McMeekin, T. A.(1998).Psychroflexus torquis gen. nov., sp. nov., a psychrophilic species from Antarctic sea ice, and reclassification of Flavobacterium gondwanense ( Dobson et al. 1993 ) as Psychroflexus gondwanense gen. nov., comb. nov. Microbiology 144, 1601–1609.[CrossRef] [Google Scholar]
  3. Chen, Y.-G., Cui, X.-L., Pukall, R., Li, H.-M., Yang, Y.-L., Xu, L.-H., Wen, M.-L., Peng, Q. & Jiang, C.-L.(2007).Salinicoccus kunmingensis sp. nov., a moderately halophilic bacterium isolated from a salt mine in Yunnan, southwest China. Int J Syst Evol Microbiol 57, 2327–2332.[CrossRef] [Google Scholar]
  4. Chen, Y.-G., Cui, X.-L., Zhang, Y.-Q., Li, W.-J., Wang, Y.-X., Kim, C.-J., Lim, J.-M., Xu, L.-H. & Jiang, C.-L.(2008).Salinimicrobium terrae sp. nov., isolated from saline soil, and emended description of the genus Salinimicrobium. Int J Syst Evol Microbiol 58, 2501–2504.[CrossRef] [Google Scholar]
  5. Cowan, S. T. & Steel, K. J.(1965).Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  6. Cui, X.-L., Mao, P.-H., Zeng, M., Li, W.-J., Zhang, L.-P., Xu, L.-H. & Jiang, C.-L.(2001).Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363. [Google Scholar]
  7. Dobson, S. J., Colwell, R. R., McMeekin, T. A. & Franzmann, P. D.(1993). Direct sequencing of the polymerase chain reaction-amplified 16S rRNA gene of Flavobacterium gondwanense sp. nov. and Flavobacterium salegens sp. nov., two new species from a hypersaline Antarctic lake. Int J Syst Bacteriol 43, 77–83.[CrossRef] [Google Scholar]
  8. Donachie, S. P., Bowman, J. P. & Alam, M.(2004).Psychroflexus tropicus sp. nov., an obligately halophilic Cytophaga-Flavobacterium-Bacteroides group bacterium from an Hawaiian hypersaline lake. Int J Syst Evol Microbiol 54, 935–940.[CrossRef] [Google Scholar]
  9. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  10. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  11. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  12. Felsenstein, J.(1993).phylip (phylogenetic inference package), version 3.5. Distributed by the author. Department of Genetics, University of Washington, Seattle, USA.
  13. Gregersen, T.(1978). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef] [Google Scholar]
  14. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef] [Google Scholar]
  15. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P. & Ward, J. M.(1985). Preparation of chromosomal, plasmid and phage DNA. In Genetic Manipulation of Streptomyces – a Laboratory Manual, pp. 79–80. Norwich, UK: F. Crowe & Sons.
  16. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  17. Kluge, A. G. & Farris, J. S.(1969). Quantitative phyletics and the evolution of anurans. Syst Zool 18, 1–32.[CrossRef] [Google Scholar]
  18. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  19. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  20. Lim, J.-M., Jeon, C. O., Song, S. M. & Kim, C.-J.(2005).Pontibacillus chungwhensis gen. nov., sp. nov., a moderately halophilic Gram-positive bacterium from a solar saltern in Korea. Int J Syst Evol Microbiol 55, 165–170.[CrossRef] [Google Scholar]
  21. Mandel, M. & Marmur, J.(1968). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. Methods Enzymol 12B, 195–206. [Google Scholar]
  22. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  23. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE: MIDI Inc.
  24. Schmit, K., Connor, A. & Britton, G.(1994). Chapter 11. Analysis of pigments: carotenoids and related polyenes. In Chemical Methods in Prokaryotic Systematics, pp. 403–462. Edited by M. Goodfellow & A. G. O'Donnell. New York: John Wiley & Sons.
  25. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  26. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24, 4876–4882. [Google Scholar]
  28. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]

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vol. , part 3, pp. 569 - 573

Fatty acid compositions of strain YIM-C238 ( sp. nov.) and other species. [PDF](51 KB)

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