1887

Abstract

A Gram-positive-staining, filamentous bacterial strain that developed cylindrical sporangia containing four oval- to rod-shaped spores at the ends of short sporangiophores on branched aerial mycelium was isolated from tropical rainforest soil near a hot spring. The cell-wall peptidoglycan contained -diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; the whole-cell hydrolysate contained rhamnose, madurose, glucose, galactose and 3--methylmannose as whole-cell sugars. The predominant menaquinone was MK-9(H). Mycolic acids were not detected. The diagnostic phospholipid was phosphatidylethanolamine. The predominant cellular fatty acids were iso-C and 10-methylated C. The G+C content of the DNA was 71.1 mol%. The phenotypic and chemotaxonomic analyses showed that the isolate had characteristics typical of members of the genus . Furthermore, 16S rRNA gene sequence analysis also indicated that this strain belongs to the genus , but as a putative novel species. Following phenotypic, chemotaxonomic and genotypic studies, the isolate is proposed to be a representative of a novel species, to be named sp. nov. The type strain is BCC 21825 (=NBRC 104271). An emended description of the genus is also presented.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.003228-0
2009-05-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/5/992.html?itemId=/content/journal/ijsem/10.1099/ijs.0.003228-0&mimeType=html&fmt=ahah

References

  1. Arai, T. ( 1975; ). Culture Media for Actinomycetes. Tokyo: Society for Actinomycetes Japan (in Japanese).
  2. Brosius, J., Dull, T. J., Sleeter, D. D. & Noller, H. F. ( 1981; ). Gene organization and primary structure of a ribosomal RNA operon from Escherichia coli. J Mol Biol 148, 107–127.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N. ( 1974; ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef]
    [Google Scholar]
  7. Hayakawa, M., Sadakata, T., Kajiura, T. & Nonomura, H. ( 1991; ). New methods for the highly selective isolation of Micromonospora and Microbispora from soil. J Ferment Bioeng 72, 320–326.[CrossRef]
    [Google Scholar]
  8. Itoh, T., Kudo, T., Parenti, F. & Seino, A. ( 1989; ). Amended description of the genus Kineosporia, based on chemotaxonomic and morphological studies. Int J Syst Bacteriol 39, 168–173.[CrossRef]
    [Google Scholar]
  9. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  10. Katsura, K., Kawasaki, H., Potacharoen, W., Saono, S., Seki, T., Yamada, Y., Uchimura, T. & Komagata, K. ( 2001; ). Asaia siamensis sp. nov., an acetic acid bacterium in the α-Proteobacteria. Int J Syst Evol Microbiol 51, 559–563.
    [Google Scholar]
  11. Kawamoto, I., Oka, T. & Nara, T. ( 1981; ). Cell wall composition of Micromonospora olivoasterospora, Micromonospora sagamiensis, and related organisms. J Bacteriol 146, 527–534.
    [Google Scholar]
  12. Kawasaki, H., Hoshino, Y., Hirata, A. & Yamasato, K. ( 1993; ). Is intracytoplasmic membrane structure a generic criterion? It does not coincide with phylogenetic interrelationships among photosynthetic purple non-sulfur bacteria. Arch Microbiol 160, 358–362.
    [Google Scholar]
  13. Kelly, K. L. ( 1964; ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  14. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  15. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
  16. Kudo, T., Itoh, T., Miyadoh, S., Shomura, T. & Seino, A. ( 1993; ). Herbidospora gen. nov., a new genus of the family Streptosporangiaceae Goodfellow et al. 1990. Int J Syst Bacteriol 43, 319–328.[CrossRef]
    [Google Scholar]
  17. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  18. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  19. Mikami, H. & Ishida, Y. ( 1983; ). Post-column fluorometric detection of reducing sugar in high-performance liquid chromatography using arginine. Bunseki Kagaku 32, E207–E210.[CrossRef]
    [Google Scholar]
  20. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  21. Nonomura, H. & Ohara, Y. ( 1957; ). Distribution of actinomycetes in the soil. II. Microbispora, a new genus of the Streptomycetaceae. J Ferment Technol 35, 307–311.
    [Google Scholar]
  22. Runmao, H., Guizhen, W. & Junying, L. ( 1993; ). A new genus of actinomycetes, Planotetraspora gen. nov. Int J Syst Bacteriol 43, 468–470.[CrossRef]
    [Google Scholar]
  23. Saito, H. & Miura, K. ( 1963; ). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef]
    [Google Scholar]
  24. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  25. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  26. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  27. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  28. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  29. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  30. Tamura, T. & Sakane, T. ( 2004; ). Planotetraspora silvatica sp. nov. and emended description of the genus Planotetraspora. Int J Syst Evol Microbiol 54, 2053–2056.[CrossRef]
    [Google Scholar]
  31. Tamura, T., Nakagaito, Y., Nishii, T., Hasegawa, T., Stackebrandt, E. & Yokota, A. ( 1994; ). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203.[CrossRef]
    [Google Scholar]
  32. Tamura, T., Suzuki, S. & Hatano, K. ( 2000; ). Acrocarpospora gen. nov., a new genus of the order Actinomycetales. Int J Syst Evol Microbiol 50, 1163–1171.[CrossRef]
    [Google Scholar]
  33. Thiemann, J. E., Pagani, H. & Beretta, G. ( 1968; ). A new genus of the Actinomycetales: Microtetraspora gen. nov. J Gen Microbiol 50, 295–303.[CrossRef]
    [Google Scholar]
  34. Uchida, K. & Aida, K. ( 1984; ). An improved method for the glycolate test for simple identification of acyl type of bacterial cell walls. J Gen Appl Microbiol 30, 131–134.[CrossRef]
    [Google Scholar]
  35. Verlander, C. P. ( 1992; ). Detection of horseradish peroxidase by colorimetry. In Nonisotopic DNA Probe Techniques, pp. 185–201. Edited by L. J. Kricka. New York: Academic Press.
  36. Williams, S. T. & Cross, T. ( 1971; ). Actinomycetes. Methods Microbiol 4, 295–334.
    [Google Scholar]
  37. Yamada, Y., Katsura, K., Kawasaki, K., Widyastuti, Y., Saono, S., Seki, T., Uchimura, T. & Komagata, K. ( 2000; ). Asaia bogorensis gen. nov., sp. nov., an unusual acetic acid bacterium in the α-Proteobacteria. Int J Syst Evol Microbiol 50, 823–829.[CrossRef]
    [Google Scholar]
  38. Yukphan, P., Potacharoen, W., Tanasupawat, S., Tanticharoen, M. & Yamada, Y. ( 2004; ). Asaia krungthepensis sp. nov., an acetic acid bacterium in the alpha-Proteobacteria. Int J Syst Evol Microbiol 54, 313–316.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.003228-0
Loading
/content/journal/ijsem/10.1099/ijs.0.003228-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 992 – 997

Cultural characteristics of sp. nov., and

Physiological characteristics of sp. nov., and

[ Single PDF file] (46 KB)



PDF

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error