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Volume 59, Issue 4

sp. nov., a tyrosinase-producing bacterium isolated from seawater Free

Abstract

An aerobic, gliding, yellow-pigmented bacterium lacking flagella and showing strong tyrosinase activity, designated strain EM41, was isolated from seawater on the eastern coast of Jeju Island in Korea. Growth was observed at 15–35 °C (optimum, 25–30 °C) and at pH 6.5–9.0 (optimum, pH 7.0–8.5). Cells were Gram-negative, negative for flexirubin pigments and catalase- and oxidase-positive. The G+C content of the genomic DNA was 33.5 mol% and the major respiratory quinone was menaquinone-6 (MK-6). Comparative 16S rRNA gene sequence analysis showed that strain EM41 formed a distinct phyletic line within the genus with a 100 % bootstrap value and was most closely related to KMM 3664 (97.0 % sequence similarity). The level of DNA–DNA relatedness between strain EM41 and KMM 3664 was about 17.8 %. On the basis of phenotypic and genotypic data, strain EM41 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EM41 (=KCTC 22297=DSM 21164).

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2009-04-01
2023-05-27
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References

  1. Bernardet, J. F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  2. Bowman, J. P.(2000). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868. [Google Scholar]
  3. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  4. Felsenstein, J.(2002).phylip (phylogeny inference package) version 3.6a. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  5. Gomori, G.(1955). Preparation of buffers for use in enzyme studies. Methods Enzymol 1, 138–146. [Google Scholar]
  6. Jeon, C. O., Park, W., Ghiorse, W. C. & Madsen, E. L.(2004).Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. Int J Syst Evol Microbiol 54, 93–97.[CrossRef] [Google Scholar]
  7. Johansen, J. E., Nielsen, P. & Sjøholm, C.(1999). Description of Cellulophaga baltica gen. nov., sp. nov. and Cellulophaga fucicola gen. nov., sp. nov. and reclassification of [Cytophaga] lytica to Cellulophaga lytica gen. nov., comb. nov. Int J Syst Bacteriol 49, 1231–1240.[CrossRef] [Google Scholar]
  8. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  9. Lane, D. J.(1991). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. Chichester: Wiley.
  10. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  11. Leifson, E.(1963). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184. [Google Scholar]
  12. Nedashkovskaya, O. I., Suzuki, M., Lysenko, A. M., Snauwaert, C., Vancanneyt, M., Swings, J., Vysotskii, M. V. & Mikhailov, V. V.(2004).Cellulophaga pacifica sp. nov. Int J Syst Evol Microbiol 54, 609–613.[CrossRef] [Google Scholar]
  13. Pearson, W. R. & Lipman, D. J.(1988). Improved tools for biological sequence comparison. Proc Natl Acad Sci U S A 85, 2444–2448.[CrossRef] [Google Scholar]
  14. Reva, O. N., Vyunitskaya, V. A., Reznik, S. R., Kozachko, I. A. & Smirnov, V. V.(1995). Antibiotic susceptibility as a taxonomic characteristic of the genus Bacillus. Int J Syst Bacteriol 45, 409–411.[CrossRef] [Google Scholar]
  15. Rosselló-Mora, R. & Amann, R.(2001). The species concept for prokaryotes. FEMS Microbiol Rev 25, 39–67.[CrossRef] [Google Scholar]
  16. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  17. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  18. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  19. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
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Cellulophaga tyrosinoxydans sp. nov., a tyrosinase-producing bacterium isolated from seawater
Int J Syst Evol Microbiol 59, 654 (2009); https://doi.org/10.1099/ijs.0.003210-0
/content/journal/ijsem/10.1099/ijs.0.003210-0
/content/journal/ijsem/10.1099/ijs.0.003210-0
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vol. , part 4, pp. 654 - 657

Transmission electron micrograph showing the general morphology of negatively stained cells of strain EM41 . [ PDF] 175 KB

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