A novel, moderately halophilic, endospore-forming bacterial strain, designated Hal 1T, was isolated from a permafrost core collected from the Canadian high Arctic. The temperature for growth of strain Hal 1T was 0–30 °C with no growth observed at either −5 or 37 °C (optimum growth at about 25 °C). Strain Hal 1T was able to grow at NaCl concentrations of 0–20 % (w/v) and did not have an absolute NaCl requirement for growth; optimal growth was at 5 % (w/v) NaCl. The level of 16S rRNA gene sequence similarity between strain Hal 1T and the type strains of Virgibacillus carmonensis and Virgibacillus necropolis was 98.2 %; values with respect to the type strains of other recognized Virgibacillus species were below 96.0 %. The DNA G+C content of strain Hal 1T was 38.2 mol%. Levels of DNA–DNA relatedness between strain Hal 1T and the type strains of V. carmonensis and V. necropolis were 14.0 and 21.0 %, respectively. The major fatty acid of strain Hal 1T was anteiso-C15 : 0, consistent with species of the genus Virgibacillus. The cell-wall peptidoglycan of strain Hal 1T was type A1α and the major respiratory quinone was MK-7. On the basis of genotypic and physiological results, strain Hal 1T (=DSM 19574T=JCM 14839T) is proposed as the type strain of a novel species of the genus Virgibacillus, namely Virgibacillus arcticus sp. nov.
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International Journal of Systematic and Evolutionary
Microbiology vol.
59 , part 9, pp. 2219 - 2225
Supplementary Fig. S1. RAPD results from comparisons of
strain Hal 1
T(
V. arcticus sp. nov.) and the type strains of recognized
Virgibacillus species.
[PDF](107 KB)