A novel, moderately halophilic, endospore-forming bacterial strain, designated Hal 1, was isolated from a permafrost core collected from the Canadian high Arctic. The temperature for growth of strain Hal 1 was 0–30 °C with no growth observed at either −5 or 37 °C (optimum growth at about 25 °C). Strain Hal 1 was able to grow at NaCl concentrations of 0–20 % (w/v) and did not have an absolute NaCl requirement for growth; optimal growth was at 5 % (w/v) NaCl. The level of 16S rRNA gene sequence similarity between strain Hal 1 and the type strains of and was 98.2 %; values with respect to the type strains of other recognized species were below 96.0 %. The DNA G+C content of strain Hal 1 was 38.2 mol%. Levels of DNA–DNA relatedness between strain Hal 1 and the type strains of and were 14.0 and 21.0 %, respectively. The major fatty acid of strain Hal 1 was anteiso-C, consistent with species of the genus . The cell-wall peptidoglycan of strain Hal 1 was type A1 and the major respiratory quinone was MK-7. On the basis of genotypic and physiological results, strain Hal 1 (=DSM 19574=JCM 14839) is proposed as the type strain of a novel species of the genus , namely sp. nov.


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vol. , part 9, pp. 2219 - 2225

RAPD results from comparisons of strain Hal 1 ( sp. nov.) and the type strains of recognized species. [PDF](107 KB)


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