Gram-positive-staining, anaerobic, non-spore-forming, lactate- and acetate-producing bacterial strains were isolated from the digestive tracts of different bumblebee species (, and ). All of the isolates produced fructose-6-phosphate phosphoketolase activity. A representative strain, BluCI/TP, was characterized further. Cells of strain BluCI/TP showed occasional bifurcation and irregular constrictions. The bacterium utilized a wide range of carbohydrates. Glucose was fermented to acetate and lactate. The DNA base composition was 47.2 mol% G+C. Complete 16S rRNA and partial gene sequences were obtained and phylogenetic relationships were determined. Strain BluCI/TP and related isolates were located in the actinobacterial cluster and were closely related to the genera , , and . The results presented support the proposal of a novel species to accommodate strain BluCI/TP, with the name sp. nov.; the type strain is BluCI/TP (=DSM 19703 =ATCC BAA-1567).


Article metrics loading...

Loading full text...

Full text loading...



  1. Barcenilla, A., Pryde, S. E., Martin, J. C., Duncan, S. H., Stewart, C. S., Henderson, C. & Flint, H. J.(2000). Phylogenetic relationship of butyrate-producing bacteria from the human gut. Appl Environ Microbiol 66, 1654–1661.[CrossRef] [Google Scholar]
  2. Biavati, B. & Mattarelli, P.(2006). The family Bifidobacteriaceae. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 3, pp. 322–382. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  3. Felis, G. E. & Dellaglio, F.(2007). Taxonomy of lactobacilli and bifidobacteria. Curr Issues Intest Microbiol 8, 44–61. [Google Scholar]
  4. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  5. Forster, R. J., Teather, R. M., Gong, J. & Deng, S. J.(1996). 16S rRNA analysis of Butyrivibrio fibrisolvens: phylogenetic position and relation to butyrate producing anaerobic bacteria from the rumen of a short-tailed deer. Lett Appl Microbiol 23, 218–222.[CrossRef] [Google Scholar]
  6. Gilliam, M.(1997). Identification and roles of non-pathogenic microflora associated with honeybees. FEMS Microbiol Lett 155, 1–10.[CrossRef] [Google Scholar]
  7. Gregg, K., Cooper, C. L., Schafer, D. J., Sharpe, H., Beard, C. E., Allen, G. & Xu, J.(1994). Detoxification of the plant toxin fluoroacetate by a genetically modified rumen bacterium. Biotechnology 12, 1361–1365.[CrossRef] [Google Scholar]
  8. Hall, T. A.(1999). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98. [Google Scholar]
  9. Huys, G., Vancanneyt, M., D'Haene, K., Falsen, E., Wauters, G. & Vandamme, P.(2007).Alloscardovia omnicolens gen. nov., sp nov., from human clinical samples. Int J Syst Evol Microbiol 57, 1442–1446.[CrossRef] [Google Scholar]
  10. Jian, W. & Dong, X.(2002). Transfer of Bifidobacterium inopinatum and Bifidobacterium denticolens to Scardovia inopinata gen. nov., comb. nov., and Parascardovia denticolens gen. nov., comb. nov., respectively. Int J Syst Evol Microbiol 52, 809–812.[CrossRef] [Google Scholar]
  11. Jian, W., Zhu, L. & Dong, X.(2001). New approach to phylogenetic analysis of the genus Bifidobacterium based on partial HSP60 gene sequences. Int J Syst Evol Microbiol 51, 1633–1638.[CrossRef] [Google Scholar]
  12. Kačániová, M., Chlebo, R., Kopernický, M. & Trakovická, A.(2004). Microflora of the honeybee gastrointestinal tract. Folia Microbiol (Praha) 49, 169–171.[CrossRef] [Google Scholar]
  13. Maidak, B. L., Larsen, N., McCaughey, M. J., Overbeek, R., Olsen, G. J., Fogel, K., Blandy, J. & Woese, C. R.(1994). The Ribosomal Database Project. Nucleic Acids Res 22, 3485–3487.[CrossRef] [Google Scholar]
  14. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  15. Mohr, K. I. & Tebbe, C. C.(2006). Diversity and phylotype consistency of bacteria in the guts of three bee species (Apoidea) at an oilseed rape field. Environ Microbiol 8, 258–272.[CrossRef] [Google Scholar]
  16. Moore, W. E. C.(1994). Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic gram-negative bacilli. Int J Syst Bacteriol 44, 338–347.[CrossRef] [Google Scholar]
  17. Posada, D. & Crandall, K. A.(1998). Modeltest: testing the model of DNA substitution. Bioinformatics 14, 817–818.[CrossRef] [Google Scholar]
  18. Rada, V. & Petr, J.(2000). A new selective medium for the isolation of glucose nonfermenting bifidobacteria from hen caeca. J Microbiol Methods 43, 127–132.[CrossRef] [Google Scholar]
  19. Sakata, S., Ryu, C. S., Kitahara, M., Sakamoto, M., Hayashi, H., Fukuyama, M. & Benno, Y.(2006). Characterization of the genus Bifidobacterium by automated ribotyping and 16S rRNA gene sequences. Microbiol Immunol 50, 1–10.[CrossRef] [Google Scholar]
  20. Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  21. Sanders, S. K., Alexander, E. L. & Braylan, R. C.(1975). A high-yield technique for preparing cells fixed in suspension for scanning electron microscopy. J Cell Biol 67, 476–480.[CrossRef] [Google Scholar]
  22. Simpson, P. J., Ross, R. P., Fitzgerald, G. F. & Stanton, C.(2004).Bifidobacterium psychraerophilum sp. nov. and Aeriscardovia aeriphila gen. nov., sp. nov., isolated from a porcine caecum. Int J Syst Evol Microbiol 54, 401–406.[CrossRef] [Google Scholar]
  23. Suutari, M. & Laakso, S.(1994). Microbial fatty-acids and thermal adaptation. Crit Rev Microbiol 20, 285–328.[CrossRef] [Google Scholar]
  24. Swofford, D. L.(2003).paup: phylogenetic analysis using parsimony (and other methods), version 4. Sunderland, MA: Sinauer Associates.
  25. Tavaré, S.(1986). Some probabilistic and statistical problems in the analysis of DNA sequences. In Some Mathematical Questions in Biology: DNA Sequence Analysis (Lectures on Mathematics in the Life Sciences, vol. 17), pp. 57–86. Edited by R. M. Miura. Providence, RI: American Mathematical Society.
  26. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  27. Van Den Velde, S., Lagrou, K., Desmet, K., Wauters, G. & Verhaegen, J.(2006). Species identification of corynebacteria by cellular fatty acid analysis. Diagn Microbiol Infect Dis 54, 99–104.[CrossRef] [Google Scholar]
  28. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J.(1991). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697–703. [Google Scholar]

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error