A novel actinomycete, strain 04-5195, that produces amicoumacin B, which targets bone morphogenetic protein-2, was isolated from a soil sample collected in Jinan, Shandong Province, China. Strain 04-5195 had morphological, biochemical, physiological and chemotaxonomic properties that were consistent with its classification in the genus and it formed a phyletic line in the 16S rRNA gene tree. It was evident from the phylogenetic data that strain 04-5195 was most closely associated with N2-11. However, the two organisms were distinguishable from one another using DNA–DNA relatedness and phenotypic data. The isolate was readily differentiated from other related strains by a set of phenotypic properties and by its phylogenetic position. Therefore, it is proposed that the isolate represents a novel species in the genus , sp. nov.; the type strain is 04-5195 (=CGMCC 4.3508 =DSM 45048).


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Scanning electron micrograph of mycelium of strain 04-5195 grown on yeast extract-malt extract agar (ISP 2) for 7 days at 28 °C. Bar, 2 µm.


Extended neighbour-joining tree derived from aligned 16S rRNA gene sequences, showing the position of strain 04-5195 within the genus . [PDF](23 KB)

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