A novel polycyclic aromatic hydrocarbon (PAH)-degrading bacterium, strain H25, which was isolated from deep-sea water of the Indian Ocean, was studied phenotypically, genotypically and phylogenetically. Strain H25 can utilize several PAHs including phenanthrene and fluoranthene as sole carbon sources. The 16S rRNA gene sequence of strain H25 showed the highest similarity with that of TUT562 (96.3 %), and showed lower similarities (92.1–96.0 %) with other members of the genus . The major fatty acids of strain H25 were C 2-OH (3.2 %), C (13.6 %), C 7 (5.2 %), C (13.4 %) and C 7 (57.0 %), which accounted for 92.3 % of the total fatty acids. It had ubiquinone 10 as the major respiratory quinone and spermidine as the major polyamine. All these characteristics were consistent with those of recognized species. Results of DNA–DNA hybridization experiments and BOX-PCR fingerprint comparisons also indicate that strain H25 represents a novel species, for which the name sp. nov. is proposed. The type strain is H25 (=MCCC 1A01080 =CGMCC 1.6784 =LMG 24713).


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rep-PCR comparision of strain H25 and related type strains. Lanes: 1, strain H25 ; 2, DSM 18518 ; 3, US6-1 ; 4, KF1 ; M, markers.


Transmission electron micrograph of negatively stained cells of strain H25 grown on 216L agar for 24 h.


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