A single strain (designated Bejo-Iso9) was isolated from a soil sample taken in Johor, Malaysia. The isolate showed motility and morphology typical of the genus under dark-field microscopy. Cells were found to be 10–13 μm in length and 0.2 μm in diameter, with a wavelength of 0.5 μm and an amplitude of approximately 0.2 μm. Phenotypically, strain Bejo-Iso9 grew in Ellinghausen–McCullough–Johnson–Harris medium at 13, 30 and 37 °C, and also in the presence of 8-azaguanine. Serologically, strain Bejo-Iso9 produced titres towards several members of the Tarassovi serogroup, but was found to be serologically unique by cross-agglutinin absorption test and thus represented a novel serovar. The proposed name for this serovar is Malaysia. Phylogenetic analysis of 16S rRNA gene sequences placed this novel strain within the radiation of the genus , with sequence similarities within the range 90.4–99.5% with respect to recognized species. DNA–DNA hybridization against the three most closely related species was used to confirm the results of the 16S rRNA gene sequence analysis. The G+C content of the genome of strain Bejo-Iso9 was 36.2 mol%. On the basis of phenotypic, serological and phylogenetic data, strain Bejo-Iso9 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Bejo-Iso9 (=WHO LT1101=KIT Bejo-Iso9).


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vol. , part 4, pp. 705 – 708

Results of serovar Malaysia strain Bejo-Iso9 culture and antiserum against serogroup Tarassovi cultures and antiserum

Similarity matrix (%) of in comparison to previously described species using 16S rRNA, and gene sequence analysis

Results of DNA–DNA hybridization of serovar Malaysia strain Bejo-Iso9 with related pathogenic species

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