@article{mbs:/content/journal/ijsem/10.1099/ijs.0.002725-0, author = "Finster, Kai Waldemar and Herbert, Rodney Andrew and Lomstein, Bente Aagaard", title = "Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov., isolated from a high Arctic permafrost soil, and emended description of the genus Spirosoma", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2009", volume = "59", number = "4", pages = "839-844", doi = "https://doi.org/10.1099/ijs.0.002725-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.002725-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", abstract = "Two pigmented, Gram-negative, non-motile, pleomorphic rod-shaped bacteria (strains SPM-9T and SPM-10T) were isolated from a permafrost soil collected from the Adventdalen valley, Spitsbergen, northern Norway. A third isolate (strain M5-H2) was recovered from the same soil sample after the sample had been exposed to simulated Martian environmental conditions. The three strains were characterized taxonomically by using a polyphasic approach. Phylogenetic, chemotaxonomic, physiological and morphological analyses demonstrated that the three isolates were most closely related to members of the genus Spirosoma. 16S rRNA gene sequence data indicated that the three isolates could be divided into two clusters: (i) strain SPM-9T and (ii) strains SPM-10T and M5-H2. This grouping was confirmed by DNA–DNA hybridization experiments. Strains SPM-9T and SPM-10T exhibited 92 % 16S rRNA gene sequence similarity to both Spirosoma linguale LMG 10896T and Spirosoma rigui WPCB 118T. The major fatty acids present in all three isolates were summed feature 3 (comprising iso-C15:0 2-OH and/or C16 : 1 ω7c; 43.0–48.2 % of the total), C16 : 1 ω5c (19.1–21.3 %), C16 : 0 (6.7–7.3 %), iso-C17 : 0 3-OH (4.7–6.0 %) and iso-C15 : 0 (2.6–5.7 %). On the basis of their phenotypic and genotypic characteristics, the new strains are assigned to two novel species of the genus Spirosoma, for which the names Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov. are proposed. The type strain of Spirosoma spitsbergense is SPM-9T (=NCIMB 14407T=DSM 19989T) and the type strain of Spirosoma luteum is SPM-10T (=NCIMB 14406T=DSM 19990T). An emended description of the genus Spirosoma is also proposed.", }