1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 4

sp. nov. and sp. nov., isolated from a high Arctic permafrost soil, and emended description of the genus Free

Abstract

Two pigmented, Gram-negative, non-motile, pleomorphic rod-shaped bacteria (strains SPM-9 and SPM-10) were isolated from a permafrost soil collected from the Adventdalen valley, Spitsbergen, northern Norway. A third isolate (strain M5-H2) was recovered from the same soil sample after the sample had been exposed to simulated Martian environmental conditions. The three strains were characterized taxonomically by using a polyphasic approach. Phylogenetic, chemotaxonomic, physiological and morphological analyses demonstrated that the three isolates were most closely related to members of the genus . 16S rRNA gene sequence data indicated that the three isolates could be divided into two clusters: (i) strain SPM-9 and (ii) strains SPM-10 and M5-H2. This grouping was confirmed by DNA–DNA hybridization experiments. Strains SPM-9 and SPM-10 exhibited 92 % 16S rRNA gene sequence similarity to both LMG 10896 and WPCB 118. The major fatty acids present in all three isolates were summed feature 3 (comprising iso-C 2-OH and/or C 7; 43.0–48.2 % of the total), C 5 (19.1–21.3 %), C (6.7–7.3 %), iso-C 3-OH (4.7–6.0 %) and iso-C (2.6–5.7 %). On the basis of their phenotypic and genotypic characteristics, the new strains are assigned to two novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. The type strain of is SPM-9 (=NCIMB 14407=DSM 19989) and the type strain of is SPM-10 (=NCIMB 14406=DSM 19990). An emended description of the genus is also proposed.

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.002725-0
2009-04-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/4/839.html?itemId=/content/journal/ijsem/10.1099/ijs.0.002725-0&mimeType=html&fmt=ahah

References

  1. Baik, K. S., Kim, M. S., Park, S. C., Lee, D. W., Lee, S. D., Ka, J.-O., Choi, S. K. & Seong, C. N.(2007).Spirosoma rigui sp. nov., isolated from fresh water. Int J Syst Evol Microbiol 57, 2870–2873.[CrossRef] [Google Scholar]
  2. Barrow, G. I. & Feltham, R. K. A.(1993).Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
  3. Bauer, A. W., Kirkby, W. M. M., Sherris, J. C. & Turck, M.(1966). Antibiotic susceptibility testing by a standardized disk method. Am J Clin Pathol 45, 493–496. [Google Scholar]
  4. Bernardet, J.-F., Nakagawa, Y. & Holmes, B.(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 52, 1049–1070.[CrossRef] [Google Scholar]
  5. Bowman, J. P.(2000). Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae and reclassification of Cytophaga uliginosa (Zobell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50, 1861–1868. [Google Scholar]
  6. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  7. Garrity, G. M. & Holt, J. G.(2001). The road map to the manual. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 119–166. Edited by D. R. Boone, R. W. Castenholz & G. M. Garrity. New York: Springer.
  8. Gosink, J. J., Woese, C. R. & Staley, J. T.(1998).Polaribacter gen. nov., with three new species, P. irgensii sp. nov., P. franzmannii sp. nov. and P. filamentus sp. nov., gas vacuolate polar marine bacteria of the Cytophaga–Flavobacterium–Bacteroidetes group and reclassification of ‘Flectobacillus glomeratus’ as Polaribacter glomeratus comb. nov. Int J Syst Bacteriol 48, 223–235.[CrossRef] [Google Scholar]
  9. Hansen, A. A., Herbert, R. A., Mikkelsen, K., Jensen, L. L., Kristoffersen, T., Tiedje, J. M., Lomstein, B. A. & Finster, K. W.(2007). Viability, diversity and composition of the bacterial community in a high Arctic permafrost soil from Spitsbergen, Northern Norway. Environ Microbiol 9, 2870–2884.[CrossRef] [Google Scholar]
  10. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  11. Jensen, L. L., Merrison, J., Hansen, A. A., Mikkelsen, K. A., Kristoffersen, P. N., Nørnberg, P., Lomstein, B. A. & Finster, K. W.(2008). A facility for long-term Mars simulation experiments: the Mars Environmental Simulation Chamber (MESCH). Astrobiology 8, 537–548.[CrossRef] [Google Scholar]
  12. Larkin, J. M. & Borrall, R.(1984). Family 1. Spirosomaceae Larkin and Borrall 1978 595AL. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 125–132. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  13. Ludwig, W., Strunk, O., Klugbauer, S., Klugbauer, N., Weizenegger, M., Neumaier, J., Bachleitner, M. & Schleifer, K.-H.(1998). Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19, 554–568.[CrossRef] [Google Scholar]
  14. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors(2004).arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef] [Google Scholar]
  15. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  16. Migula, W.(1894). Über ein neues System der Bakterien. Arb Bakt Inst Karlsruhe 1, 235–238. [Google Scholar]
  17. Pruesse, E., Quast, C., Knittel, K., Fuchs, B., Ludwig, W., Peplies, J., Glöckner, F. & Silva, O.(2007). A comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. Nucleic Acids Res 35, 7188–7196.[CrossRef] [Google Scholar]
  18. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  19. Swofford, D. L.(2003).paup*: phylogenetic analysis using parsimony (* and other methods), version 4.0b10. Sunderland, MA: Sinauer Associates.
  20. Urakami, T. & Komagata, K.(1986). Methanol-utilizing Ancylobacter strains and comparison of their cellular fatty acid compositions and quinone systems with those of Spirosoma, Flectobacillus, and Runella species. Int J Syst Bacteriol 36, 415–421.[CrossRef] [Google Scholar]
  21. Vancanneyt, M., Nedashkovskaya, O. I., Snauwaert, C., Mortier, S., Vandemeulebroecke, K., Hoste, B., Dawyndt, P., Frolova, G. M., Janssens, D. & Swings, J.(2006).Larkinella insperata gen. nov, sp. nov., a bacterium of the phylum ‘Bacteroidetes’ isolated from water of a steam generator. Int J Syst Evol Microbiol 56, 237–241.[CrossRef] [Google Scholar]
  22. Vandamme, P., Pot, B., Gillis, M., De Vos, P., Kersters, K. & Swings, J.(1996). Polyphasic taxonomy, a consensus approach to bacterial systematics. Microbiol Rev 60, 407–438. [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.002725-0
Loading
Spirosoma spitsbergense sp. nov. and Spirosoma luteum sp. nov., isolated from a high Arctic permafrost soil, and emended description of the genus Spirosoma
Int J Syst Evol Microbiol 59, 839 (2009); https://doi.org/10.1099/ijs.0.002725-0
/content/journal/ijsem/10.1099/ijs.0.002725-0
/content/journal/ijsem/10.1099/ijs.0.002725-0
Loading

Data & Media loading...

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error