1887

Abstract

A Gram-reaction-negative, non-motile, aerobic bacterium, designated HJ50, was isolated from deep seawater of the East Sea, South Korea. Cells were ovoid to rod-shaped (0.5–0.8×1.3–3.0 μm), often with unequal ends, suggesting a budding mode of reproduction. The strain had an absolute requirement for sea salts and tolerated up to 20 % (w/v) sea salts. Propionate, -lactate, 2-ketogluconate, 3-hydroxybutyrate and rhamnose were used as growth substrates, but not mannitol, salicin, 4-hydroxybenzoate or acetate. The major fatty acid was summed feature 7 (C 7/9/12) and the DNA G+C content was 59.0±0.1 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that this strain was affiliated with the genus . Similarities between the 16S rRNA gene sequence of strain HJ50 (1430 nt) and those of type strains of members of the genus were 94.1–96.3 %. DNA–DNA relatedness values between strain HJ50 and the type strains of members of the genus were low (1.3–24.6 %). Physiological and biochemical differences support assignment of strain HJ50 to the genus as a representative of a novel species. The name sp. nov. is proposed, with HJ50 (=KCTC 22224 =LMG 24468) as the type strain.

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2009-11-01
2019-10-14
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vol. , part 11, pp. 2718 - 2723

Two-dimensional TLC of polar lipids of strain HJ50 . Chloroform/methanol/water (65:25:4) was used in the first direction, followed by chloroform/acetic acid/methanol/water (80:15:12:4) in the second direction. Total polar lipids were stained with 5 % ethanolic molybdophosphoric acid. DPG, Diphosphatidylglycerol; L1–L5, unidentified lipids; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; PL1, unidentified phospholipid; PME, phosphatidylmonomethylethanolamine; PS, phosphatidylserine.



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