Three novel bacterial strains, PVAS-1, B3W22 and B8W22, were isolated from cryotubes used to collect air samples at altitudes of between 27 and 41 km. Based on phenotypic characteristics, chemotaxonomic features, DNA–DNA hybridization with the nearest phylogenetic neighbours and phylogenetic analysis based on partial 16S rRNA gene sequences (PVAS-1, 1196 nt; B3W22, 1541 nt; B8W22, 1533 nt), the three strains were identified as representing novel species, and the names proposed are sp. nov. (type strain PVAS-1 =MTCC 8307 =DSM 21601 =CCUG 56714), sp. nov. (type strain B3W22 =MTCC 7902 =JCM 13838) and sp. nov. (type strain B8W22 =MTCC 7755 =JCM 13839).


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(a) Lipids of DSM 12223 (lane 1) and sp. nov. B3W22 (lane 2) and standards of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylinositol (PI) (lanes 3–7). (b) Lipids of sp. nov. B8W22 (lane 1) and MTCC 428 (lane 2) and standards of DPG, PG and PE (lanes 3–5). In (a), UL represents an unidentified lipid.


(a) Lipids of DSM 12223 (lane 1) and sp. nov. B3W22 (lane 2) and standards of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylinositol (PI) (lanes 3–7). (b) Lipids of sp. nov. B8W22 (lane 1) and MTCC 428 (lane 2) and standards of DPG, PG and PE (lanes 3–5). In (a), UL represents an unidentified lipid.


Sensitivity of sp. nov. B3W22 , sp. nov. B8W22 , sp. nov. PVAS-1 and related type strains to UV radiation. [ PDF] 58 KB

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