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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 7

gen. nov., sp. nov., a member of the family isolated from human faeces Free

Abstract

A novel, strictly anaerobic, non-spore-forming, Gram-negative coccobacillus (strain YIT 11859) was isolated from human faeces. Biochemically, this strain was largely unreactive and was asaccharolytic. Growth of strain YIT 11859 in peptone-yeast extract broth produced no visible turbidity, and a trace amount of propionate was detected as an end product of metabolism. 16S rRNA gene sequence analysis showed that strain YIT 11859 was related most closely to the type strains of species, with 90.8–88.0 % sequence similarity. Phylogenetic analysis of these and other related sequences confirmed that strain YIT 11859 was phylogenetically most closely associated with species, but formed a separate cluster, indicating that strain YIT 11859 represents a novel member of the family . Fatty acid analysis demonstrated the presence of a high concentration of C 9 (75 % of the total). The main respiratory quinones were menaquinone (MK–6) and methylated menaquinone (MMK-6). The G+C content of the DNA was 49.8 mol%. These results suggest that strain YIT 11859 represents a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is YIT 11859 (=DSM 21040 =JCM 15078).

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Keyword(s): FAME, fatty acid methyl ester , ML, maximum likelihood and MP, maximum parsimony
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2009-07-01
2025-03-23
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Parasutterella excrementihominis gen. nov., sp. nov., a member of the family Alcaligenaceae isolated from human faeces
Int J Syst Evol Microbiol 59, 1793 (2009); https://doi.org/10.1099/ijs.0.002519-0
/content/journal/ijsem/10.1099/ijs.0.002519-0
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vol. , part 7, pp. 1793 - 1797

Cellular fatty acid compositions of strain YIT 11859 and related type species in the family .

Maximum-likelihood (Fig. S1) and maximum-parsimony (Fig. S2) phylogenetic trees based on 16S rRNA gene sequences, showing the nearest neighbours of gen. nov., sp. nov. YIT 11859 within the family .

[PDF file of Supplementary Table and Figures](135 KB)

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