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Abstract

A novel non-sporulating, non-motile, catalase- and oxidase-positive, facultatively anaerobic, moderately halophilic, Gram-positive coccus, designated JSM 076033, was isolated from a sea urchin () collected from the South China Sea. Strain JSM 076033 was able to grow in the presence of 0.5–25.0 % (w/v) total salts and at pH 6.0–10.0 and 10–45 °C; optimum growth was observed with 5.0–10.0 % (w/v) total salts and at pH 7.0–8.0 and 25–30 °C. The major amino acid constituents of the cell wall were glycine, lysine and alanine. The major cellular fatty acids were anteiso-C, iso-C and anteiso-C. The respiratory quinones were MK-7 (60.7 %) and MK-6 (39.3 %) and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unidentified phospholipid. The DNA G+C content was 40.3 mol%. A phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain JSM 076033 should be assigned to the genus . The sequence similarities between the novel isolate and the type strains of recognized species were in the range 95.2–97.2 %. The results of the phylogenetic analysis, combined with DNA–DNA relatedness data, phenotypic characteristics and chemotaxonomic information, support the view that strain JSM 076033 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JSM 076033 (=CCTCC AA 207028 =DSM 19772 =KCTC 13189).

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2009-07-01
2019-09-15
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Fatty acid compositions (%) of strain JSM 076033 and members of the genus [ PDF] (50 KB)



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