An aerobic, pink-pigmented, budding and rosette-forming bacterium was isolated from an acidic peat bog and designated strain A10. The 16S rRNA gene sequence analysis showed that strain A10 was a member of the order and belonged to a phylogenetic lineage defined by the genus , with 90 % sequence similarity to that of , the only taxonomically described organism of this group. Ellipsoid-shaped cells of strain A10 were uniformly covered with crateriform pits and possessed long (up to 10–15 μm) and unusually thick (0.5–0.7 μm) stalks of a unique ultrastructure. Thin sections revealed a complex intracellular membrane system compartmentalizing the cells. Strain A10 was a moderately acidophilic, mesophilic organism capable of growth at pH values between 3.8 and 7.2 (with an optimum at pH 5.5–6.0) and at temperatures between 10 and 30 °C (with an optimum at 20–25 °C). The major fatty acids were C, C 5 and C 5 and the major quinone was MK-6. Cells of strain A10 contained high amounts of bound saturated and monounsaturated C–C (-1) hydroxy fatty acids. The G+C content of the DNA was 62.5 mol%. The unique cell morphology, the capability of growth in acidic conditions and a number of chemotaxonomic and genotypic characteristics served to differentiate strain A10 from . Based on these data, the novel isolate should be considered as representing a novel genus and species of planctomycetes, for which the name gen. nov., sp. nov. is proposed The type strain is A10 (=DSM 19928=VKM B-2478).


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Comparison of the phospholipid fatty acid compositions of strain A10 ( gen. nov., sp. nov.) and DSM 5831 . [PDF](77 KB)


Electron micrographs of negatively stained preparations of cells of strain A10 . [PDF](54 KB)


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