A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of the 16S rRNA gene sequence of strain A121 showed its affiliation with the group of psychrophilic and psychrotolerant members of cluster I of the genus , DSM 14206 being the closest relative (sequence similarity 98.5 %). Levels of DNA–DNA relatedness between strain A121 and the type strains of phylogenetically related species ranged from 33 to 52 %. Strain A121 grew in PY broth at temperatures between 4 and 28 °C (optimum 15–20 °C), at pH 6.0–8.0 (optimum pH 6.5–7.2) and in NaCl concentrations of 0–10.0 % (optimum 0–2.0 %). The strain utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose, fructose, trehalose, maltose and starch; it also hydrolysed gelatin. Predominant fatty acids were C 9, C 9 DMA, C and C. The DNA G+C content was 31.5 mol%. On the basis of its overall genotypic and phenotypic characteristics, strain A121 is classified within a novel species of the genus , sp. nov. The type strain is A121 (=VKM B-2369 =DSM 17763).


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vol. , part 6, pp. 1421 - 1426

Growth of strain A121 at various incubation temperatures and NaCl concentrations (at 15 °C).

Extended dendrogram demonstrating the position of strain A121 ( sp. nov.) among closely related species of the genus , based on 16S rRNA gene sequence phylogenetic analysis.

Whole-cell fatty acid profiles of strain A121 and strains of related species.

[PDF file of Supplementary Figs and Table](175 KB)


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