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Abstract

A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of the 16S rRNA gene sequence of strain A121 showed its affiliation with the group of psychrophilic and psychrotolerant members of cluster I of the genus , DSM 14206 being the closest relative (sequence similarity 98.5 %). Levels of DNA–DNA relatedness between strain A121 and the type strains of phylogenetically related species ranged from 33 to 52 %. Strain A121 grew in PY broth at temperatures between 4 and 28 °C (optimum 15–20 °C), at pH 6.0–8.0 (optimum pH 6.5–7.2) and in NaCl concentrations of 0–10.0 % (optimum 0–2.0 %). The strain utilized a narrow range of carbohydrates as sources of carbon and energy, including glucose, fructose, trehalose, maltose and starch; it also hydrolysed gelatin. Predominant fatty acids were C 9, C 9 DMA, C and C. The DNA G+C content was 31.5 mol%. On the basis of its overall genotypic and phenotypic characteristics, strain A121 is classified within a novel species of the genus , sp. nov. The type strain is A121 (=VKM B-2369 =DSM 17763).

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2009-06-01
2019-10-21
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References

  1. Benson, D. A., Boguski, M. S., Lipman, D. J., Ostell, J., Oullette, B. F. F., Rapp, B. A. & Wheeler, D. L. ( 1999; ). GenBank. Nucleic Acids Res 27, 12–17.[CrossRef]
    [Google Scholar]
  2. Broda, D. M., Lawson, P. A., Bell, R. G. & Musgrave, D. R. ( 1999; ). Clostridium frigidicarnis sp. nov., a psychrotolerant bacterium associated with ‘blown pack’ spoilage of vacuum-packed meats. Int J Syst Bacteriol 49, 1539–1550.[CrossRef]
    [Google Scholar]
  3. Collins, M. D., Rodrigues, U. M., Dainty, R. H., Edwards, R. A. & Roberts, T. A. ( 1992; ). Taxonomic studies on a psychrophilic Clostridium from vacuum-packed beef: description of Clostridium estertheticum sp. nov. FEMS Microbiol Lett 75, 235–240.
    [Google Scholar]
  4. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Hippe, H. & Farrow, J. A. E. ( 1994; ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812–826.[CrossRef]
    [Google Scholar]
  5. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  6. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  7. Garrity, G. M., Bell, J. A. & Lilburn, T. G. ( 2004; ). Taxonomic outline of the prokaryotes. In Bergey's Manual of Systematic Bacteriology, 2nd edn, release 5.0. New York: Springer.
  8. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  9. Gilichinsky, D., Rivkina, E., Bakermans, C., Shcherbakova, V., Petrovskaya, L., Ozerskaya, S., Ivanushkina, N., Kochkina, G., Laurinavichuis, K. & other authors ( 2005; ). Biodiversity of cryopegs in permafrost. FEMS Microbiol Ecol 53, 117–128.[CrossRef]
    [Google Scholar]
  10. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  11. Hippe, H., Andreesen, J. R. & Gottschalk, G. ( 1992; ). The genus Clostridium - nonmedical. In The Prokaryotes, 2nd edn, pp. 1800–1866. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  12. Hohorst, H. J. ( 1970; ). (+)-Lactate. In Methods of Enzymatic Analysis, vol. 2, p. 1425. Weinhein: Verlag Chemie.
  13. Hungate, R. E. ( 1969; ). A roll tube method for cultivation of strict anaerobes. Methods Microbiol 3B, 117–132.
    [Google Scholar]
  14. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  15. Kalchayanand, N., Ray, B. & Field, R. A. ( 1993; ). Characteristics of psychrotrophic Clostridium laramie causing spoilage of vacuum-packaged refrigerated fresh and roasted beef. J Food Prot 56, 13–17.
    [Google Scholar]
  16. Lawson, P., Dainty, R. H., Kristiansen, N., Berg, J. & Collins, M. D. ( 1994; ). Characterization of a psychrotrophic Clostridium causing spoilage in vacuum-packed cooked pork: description of Clostridium algidicarnis sp. nov. Lett Appl Microbiol 19, 153–157.[CrossRef]
    [Google Scholar]
  17. Maidak, B. L., Cole, J. R., Lilburn, T. G., Parker, C. T., Jr, Saxman, P. R., Farris, R. J., Garrity, G. M., Olsen, G. J., Schmidt, T. M. & Tiedje, J. M. ( 2001; ). The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29, 173–174.[CrossRef]
    [Google Scholar]
  18. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  19. Morita, R. Y. ( 1975; ). Psychrophilic bacteria. Bacteriol Rev 39, 144–167.
    [Google Scholar]
  20. Mountfort, D. O., Rainey, F. A., Burghardt, J., Kaspar, H. F. & Stackebrandt, E. ( 1997; ). Clostridium vincentii sp. nov., a new obligately anaerobic, saccharolytic, psychrophilic bacterium isolated from low-salinity pond sediment of the McMurdo Ice Shelf, Antarctica. Arch Microbiol 167, 54–60.[CrossRef]
    [Google Scholar]
  21. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  22. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  23. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  24. Shcherbakova, V. A., Chuvilskaya, N. A., Rivkina, E. M., Pecheritsyna, S. A., Laurinavichuis, K. S., Suzina, N. E., Osipov, G. A., Lysenko, A. M., Gilichinsky, D. A. & Akimenko, V. K. ( 2005; ). Novel psychrophilic aerobic spore-forming bacterium from the overcooled water brine in permafrost: description Clostridium algoriphilum sp. nov. Extremophiles 9, 239–246.[CrossRef]
    [Google Scholar]
  25. Shtarkman, N. B., Obraztsova, A. Ya., Laurinavichuis, K. S., Galushko, A. S. & Akimenko, V. K. ( 1995; ). Enhanced conversion of (meth)acrylic acids to methane by association of specific anaerobic microflora and granulated sludge. Microbiology (English translation of Mikrobiologiia) 64, 270–274.
    [Google Scholar]
  26. Spring, S., Merkhoffer, B., Weiss, N., Kroppenstedt, R. M., Hippe, H. & Stackebrandt, E. ( 2003; ). Characterization of novel psychrophilic clostridia from an Antarctic microbial mat: description of Clostridium frigoris sp. nov., Clostridium lacusfryxellense sp. nov., Clostridium bowmanii sp. nov. and Clostridium psychrophilum sp. nov. and reclassification of Clostridium laramiense as Clostridium estertheticum subsp. laramiense subsp. nov. Int J Syst Evol Microbiol 53, 1019–1029.[CrossRef]
    [Google Scholar]
  27. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  28. Van de Peer, Y. & De Wachter, R. ( 1994; ). treecon for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10, 569–570.
    [Google Scholar]
  29. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  30. Wiegel, J., Tanner, R. & Rainey, F. ( 2006; ). An introduction to the family Clostridiaceae. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, pp. 654–678. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
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vol. , part 6, pp. 1421 - 1426

Growth of strain A121 at various incubation temperatures and NaCl concentrations (at 15 °C).

Extended dendrogram demonstrating the position of strain A121 ( sp. nov.) among closely related species of the genus , based on 16S rRNA gene sequence phylogenetic analysis.

Whole-cell fatty acid profiles of strain A121 and strains of related species.

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