RT Journal Article SR Electronic(1) A1 Evans, Nicholas J. A1 Brown, Jennifer M. A1 Demirkan, Ibrahim A1 Murray, Richard D. A1 Birtles, Richard J. A1 Hart, C. Anthony A1 Carter, Stuart D.YR 2009 T1 Treponema pedis sp. nov., a spirochaete isolated from bovine digital dermatitis lesions JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 5 SP 987 OP 991 DO https://doi.org/10.1099/ijs.0.002287-0 PB Microbiology Society, SN 1466-5034, AB Bovine digital dermatitis (BDD) is a debilitating infection that is being increasingly recognized in livestock worldwide. Several treponeme phylotypes have been identified in BDD lesions, although only a single BDD-associated treponeme taxon (Treponema brennaborense) has been proposed thus far. In a previous study, we observed that four BDD-associated spirochaete isolates formed a distinct phylogenetic cluster on the basis of 16S rRNA gene sequence analysis and shared less than 97 % sequence similarity with recognized treponeme species. Further characterization of these isolates on the basis of growth characteristics, flaB2 gene sequences, enzyme profiles and cell morphology confirmed that they formed a coherent taxonomic group displaying marked genotypic and phenotypic differences with respect to recognized treponeme species. The four novel isolates displayed a novel 3 : 6 : 3 flagellar pattern rather than the 2 : 4 : 2 pattern shown by their closest relatives and exhibited esterase C4, esterase lipase C8, trypsin and chymotrypsin enzyme activities. Therefore these four new isolates represent a novel species of the genus Treponema, for which the name Treponema pedis sp. nov. is proposed. The type strain is T3552BT (=DSM 18691T=NCTC 13403T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.002287-0