1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 5

sp. nov., a spirochaete isolated from bovine digital dermatitis lesions Free

Abstract

Bovine digital dermatitis (BDD) is a debilitating infection that is being increasingly recognized in livestock worldwide. Several treponeme phylotypes have been identified in BDD lesions, although only a single BDD-associated treponeme taxon () has been proposed thus far. In a previous study, we observed that four BDD-associated spirochaete isolates formed a distinct phylogenetic cluster on the basis of 16S rRNA gene sequence analysis and shared less than 97 % sequence similarity with recognized treponeme species. Further characterization of these isolates on the basis of growth characteristics, gene sequences, enzyme profiles and cell morphology confirmed that they formed a coherent taxonomic group displaying marked genotypic and phenotypic differences with respect to recognized treponeme species. The four novel isolates displayed a novel 3 : 6 : 3 flagellar pattern rather than the 2 : 4 : 2 pattern shown by their closest relatives and exhibited esterase C4, esterase lipase C8, trypsin and chymotrypsin enzyme activities. Therefore these four new isolates represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is T3552B (=DSM 18691=NCTC 13403).

  • Published Online:
Keyword(s): BDD, bovine digital dermatitis and FCS, fetal calf serum
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.002287-0
2009-05-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/5/987.html?itemId=/content/journal/ijsem/10.1099/ijs.0.002287-0&mimeType=html&fmt=ahah

References

  1. Choi, B. K., Nattermann, H., Grund, S., Haider, W. & Gobel, U. B.(1997). Spirochetes from digital dermatitis lesions in cattle are closely related to treponemes associated with human periodontitis. Int J Syst Bacteriol 47, 175–181.[CrossRef] [Google Scholar]
  2. Demirkan, I., Williams, H. F., Dhawi, A., Carter, S. D., Winstanley, C., Bruce, K. D. & Hart, C. A.(2006). Characterization of a spirochaete isolated from a case of bovine digital dermatitis. J Appl Microbiol 101, 948–955.[CrossRef] [Google Scholar]
  3. Dewhirst, F. E., Tamer, M. A., Ericson, R. E., Lau, C. N., Levanos, V. A., Boches, S. K., Galvin, J. L. & Paster, B. J.(2000). The diversity of periodontal spirochetes by 16S rRNA analysis. Oral Microbiol Immunol 15, 196–202.[CrossRef] [Google Scholar]
  4. Evans, N. J., Brown, J., Demirkan, I., Murray, R. D., Vink, D., Blowey, R. W., Hart, C. A. & Carter, S. D.(2008). Three unique groups of spirochetes isolated from digital dermatitis lesions in UK cattle. Vet Microbiol 130, 141–150.[CrossRef] [Google Scholar]
  5. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  6. Nordhoff, M., Moter, A., Schrank, K. & Wieler, L. H.(2008). High prevalence of treponemes in bovine digital dermatitis – a molecular epidemiology. Vet Microbiol 131, 293–300.[CrossRef] [Google Scholar]
  7. Radolf, J. D., Hazlett, K. R. O. & Lukehart, S. A.(2006). Pathogenesis of syphilis. In Pathogenic Treponema: Molecular and Cellular Biology, pp. 197–236. Edited by J. D. Radolf & S. A. Lukehart. Norfolk, UK: Caister Academic Press.
  8. Schrank, K., Choi, B. K., Grund, S., Moter, A., Heuner, K., Nattermann, H. & Gobel, U. B.(1999).Treponema brennaborense sp. nov., a novel spirochaete isolated from a dairy cow suffering from digital dermatitis. Int J Syst Bacteriol 49, 43–50.[CrossRef] [Google Scholar]
  9. Smibert, R. M.(1984). Genus III Treponema. In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 49–57. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams and Wilkins.
  10. Thompson, J. D., Higgins, D. G. & Gibson, T. J.(1994).clustalw: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef] [Google Scholar]
  11. Trott, D. J., Moeller, M. R., Zuerner, R. L., Goff, J. P., Waters, W. R., Alt, D. P., Walker, R. L. & Wannemuehler, M. J.(2003). Characterization of Treponema phagedenis-like spirochetes isolated from papillomatous digital dermatitis lesions in dairy cattle. J Clin Microbiol 41, 2522–2529.[CrossRef] [Google Scholar]
  12. Walker, R. L., Read, D. H., Loretz, K. J. & Nordhausen, R. W.(1995). Spirochetes isolated from dairy cattle with papillomatous digital dermatitis and interdigital dermatitis. Vet Microbiol 47, 343–355.[CrossRef] [Google Scholar]
  13. Wyss, C., Choi, B. K., Schüpbach, P., Guggenheim, B. & Göbel, U. B.(1997).Treponema amylovorum sp. nov., a saccharolytic spirochete of medium size isolated from an advanced human periodontal lesion. Int J Syst Bacteriol 47, 842–845.[CrossRef] [Google Scholar]
  14. Wyss, C., Moter, A., Choi, B. K., Dewhirst, F. E., Xue, Y., Schüpbach, P., Göbel, U. B., Paster, B. J. & Guggenheim, B.(2004).Treponema putidum sp. nov., a medium-sized proteolytic spirochaete isolated from lesions of human periodontitis and acute necrotizing ulcerative gingivitis. Int J Syst Evol Microbiol 54, 1117–1122.[CrossRef] [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.002287-0
Loading
Treponema pedis sp. nov., a spirochaete isolated from bovine digital dermatitis lesions
Int J Syst Evol Microbiol 59, 987 (2009); https://doi.org/10.1099/ijs.0.002287-0
/content/journal/ijsem/10.1099/ijs.0.002287-0
/content/journal/ijsem/10.1099/ijs.0.002287-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 987 - 991

Growth characteristics of strain T3552B on FAA plates supplemented with defibrinated sheep blood and FCS. [ PDF] 565 KB



PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error