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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 6

sp. nov., isolated from the gut of Free

Abstract

During a search for exo-enzyme-producing bacteria in the gut of an insect, , a novel bacterium capable of degrading pectin was isolated. The isolate, designated strain RCB-08, comprised Gram-positive, endospore-forming, motile rods capable of growth at 15–30 °C and pH 6.0–8.7. The DNA G+C content of the isolate was 51.5 mol% and the predominant cellular fatty acid was anteiso-C (74.1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RCB-08 was affiliated with a cluster within the , and was related most closely to NBRC 15376, with a sequence similarity of 96.7 %. The DNA–DNA relatedness value for strain RCB-08 with NBRC 15376 was 15.0 %. Strain RCB-08 hydrolysed pectin, but not cellulose, casein, starch or xylan. Strain RCB-08 could be clearly distinguished from other species on the basis of characteristics observed using a polyphasic approach. Therefore strain RCB-08 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RCB-08 (=KCTC 13222=CECT 7358).

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2009-06-01
2025-05-21
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References

  1. Ash, C., Priest, F. G. & Collins, M. D.(1993). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Antonie van Leeuwenhoek 64, 253–260. [Google Scholar]
  2. Breznak, J. A.(1982). Intestinal microbiota of termites and other xylophagous insects. Annu Rev Microbiol 36, 323–343.[CrossRef] [Google Scholar]
  3. Broderick, N. A., Raffa, K. F., Goodman, R. M. & Handelsman, J.(2004). Census of the bacterial community of the gypsy moth larval midgut by using culturing and culture-independent methods. Appl Environ Microbiol 70, 293–300.[CrossRef] [Google Scholar]
  4. Brune, A. & Friedrich, M.(2000). Microecology of the termite gut: structure and function on a microscale. Curr Opin Microbiol 3, 263–269.[CrossRef] [Google Scholar]
  5. Chen, D.-Q. & Purcell, A. H.(1997). Occurrence and transmission of facultative endosymbionts in aphids. Curr Microbiol 34, 220–225.[CrossRef] [Google Scholar]
  6. Collins, M. D. & Jones, D.(1981). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 45, 316–354. [Google Scholar]
  7. Daane, L. L., Harjono, I., Barns, S. M., Launen, L. A., Palleron, N. J. & Häggblom, M. M.(2002). PAH-degradation by Paenibacillus spp. and description of Paenibacillus naphthalenovorans sp. nov., a naphthalene-degrading bacterium from the rhizosphere of salt marsh plants. Int J Syst Evol Microbiol 52, 131–139. [Google Scholar]
  8. Egert, M., Stingl, U., Bruun, L. D., Pommerenke, B., Brune, A. & Friedrich, M. W.(2005). Structure and topology of microbial communities in the major gut compartments of Melolontha melolontha larvae (Coleoptera: Scarabaeidae). Appl Environ Microbiol 71, 4556–4566.[CrossRef] [Google Scholar]
  9. Felsenstein, J.(1981). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef] [Google Scholar]
  10. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  11. Heyndrickx, M., Vandemeulebroecke, K., Hoste, B., Janssen, P., Kersters, K., De Vos, P., Logan, N. A., Ali, N. & Berkeley, R. C.(1996). Reclassification of Paenibacillus (formerly Bacillus) pulvifaciens (Nakamura 1984) Ash et al. 1994, a later subjective synonym of Paenibacillus (formerly Bacillus) larvae (White 1906) Ash et al. 1994, as a subspecies of P. larvae, with emended descriptions of P. larvae as P. larvae subsp. larvae and P. larvae subsp. pulvifaciens. Int J Syst Bacteriol 46, 270–279.[CrossRef] [Google Scholar]
  12. Kämpfer, P., Rosselló-Mora, R., Falsen, E., Busse, H. J. & Tindall, B. J.(2006).Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov. Int J Syst Evol Microbiol 56, 781–786.[CrossRef] [Google Scholar]
  13. Kanzawa, Y., Harada, A., Takeuchi, M., Yokota, A. & Harada, T.(1995).Bacillus curdlanolyticus sp. nov. and Bacillus kobensis sp. nov., which hydrolyze resistant curdlan. Int J Syst Bacteriol 45, 515–521.[CrossRef] [Google Scholar]
  14. Komagata, K. & Suzuki, K.(1987). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207. [Google Scholar]
  15. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  16. Kusuda, R., Kawai, K., Salati, F., Banner, C. R. & Fryer, J. L.(1991).Enterococcus seriolicida sp. nov., a fish pathogen. Int J Syst Bacteriol 41, 406–409.[CrossRef] [Google Scholar]
  17. Lányí, B.(1987). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67. [Google Scholar]
  18. Lee, H. J., Shin, D. J., Cho, N. C., Kim, H. O., Shin, S. Y., Im, S. Y., Lee, H. B., Chum, S. B. & Bai, S.(2000). Cloning, expression and nucleotide sequences of two xylanase genes from Paenibacillus sp. Biotechnol Lett 22, 387–392.[CrossRef] [Google Scholar]
  19. Lee, J.-S., Lee, K. C., Pyun, Y.-R. & Bae, K. S.(2003).Arthrobacter koreensis sp. nov., a novel alkalitolerant bacterium from soil. Int J Syst Evol Microbiol 53, 1277–1280.[CrossRef] [Google Scholar]
  20. Maidak, B. L., Cole, J. R., Lilburn, T. G., Parker, C. T., Jr, Saxman, P. R., Farris, R. J., Garrity, G. M., Olsen, G. J., Schmidt, T. M. & Tiedje, J. M.(2001). The RDP-II (Ribosomal Database Project). Nucleic Acids Res 29, 173–174.[CrossRef] [Google Scholar]
  21. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  22. Montes, M. J., Mercade, E., Bozal, N. & Guinea, J.(2004).Paenibacillus antarcticus sp. nov., a novel psychrotolerant organism from the Antarctic environment. Int J Syst Evol Microbiol 54, 1521–1526.[CrossRef] [Google Scholar]
  23. Nakamura, L. K.(1987).Bacillus alginolyticus sp. nov. and Bacillus chondroitinus sp. nov., two alginate-degrading species. Int J Syst Bacteriol 37, 284–286.[CrossRef] [Google Scholar]
  24. Park, D.-S., Oh, H.-W., Jeong, W.-J., Kim, H., Park, H.-Y. & Bae, K. S.(2007). A culture-based study of the bacterial communities within the guts of nine longicorn beetle species and their exo-enzyme producing properties for degrading xylan and pectin. J Microbiol 45, 394–401. [Google Scholar]
  25. Paster, B. J. & Canale-Parola, E.(1982). Physiological diversity of rumen spirochetes. Appl Environ Microbiol 43, 686–693. [Google Scholar]
  26. Rivas, R., Mateos, P. F., Martínez-Molina, E. & Velázquez, E.(2005a).Paenibacillus xylanilyticus sp. nov., an airborne xylanolytic bacterium. Int J Syst Evol Microbiol 55, 405–408.[CrossRef] [Google Scholar]
  27. Rivas, R., Gutiérrez, C., Abril, A., Mateos, P. F., Martínez-Molina, E., Ventosa, A. & Velázquez, E.(2005b).Paenibacillus rhizosphaerae sp. nov., isolated from the rhizosphere of Cicer arietinum. Int J Syst Evol Microbiol 55, 1305–1309.[CrossRef] [Google Scholar]
  28. Rivas, R., García-Fraile, P., Mateos, P. F., Martínez-Molina, E. & Velázquez, E.(2006).Paenibacillus cellulosilyticus sp. nov., a cellulolytic and xylanolytic bacterium isolated from the bract phyllosphere of Phoenix dactylifera. Int J Syst Evol Microbiol 56, 2777–2781.[CrossRef] [Google Scholar]
  29. Saha, P., Mondal, A. K., Mayilraj, S., Krishnamurthi, S., Bhattacharya, A. & Chakrabarti, T.(2005).Paenibacillus assamensis sp. nov., a novel bacterium isolated from a warm spring in Assam, India. Int J Syst Evol Microbiol 55, 2577–2581.[CrossRef] [Google Scholar]
  30. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  31. Sambrook, J. & Russell, D. W.(2001).Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  32. Sánchez, M. M., Fritze, D., Blanco, A., Spröer, C., Tindall, B. J., Schumann, P., Kroppenstedt, R. M., Diaz, P. & Pastor, F. I. J.(2005).Paenibacillus barcinonensis sp. nov., a xylanase-producing bacterium isolated from a rice field in the Ebro River delta. Int J Syst Evol Microbiol 55, 935–939.[CrossRef] [Google Scholar]
  33. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  34. Shida, O., Takagi, H., Kadowaki, K., Nakamura, L. K. & Komagata, K.(1997). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. Int J Syst Bacteriol 47, 289–298.[CrossRef] [Google Scholar]
  35. Shin, Y. K., Lee, J.-S., Chun, C. O., Kim, H.-J. & Park, Y.-H.(1996). Isoprenoid quinone profiles of the Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 6, 68–69. [Google Scholar]
  36. Skipper, N., Sutherland, M., Davies, R. W., Kilburn, D., Miller, R. C., Jr, Warren, A. & Wong, R.(1985). Secretion of a bacterial cellulase by yeast. Science 230, 958–960.[CrossRef] [Google Scholar]
  37. Šmerda, J., Sedláček, I., Páčová, Z., Durnová, E., Smíšková, A. & Havel, L.(2005).Paenibacillus mendelii sp. nov., from surface-sterilized seeds of Pisum sativum L. Int J Syst Evol Microbiol 55, 2351–2354.[CrossRef] [Google Scholar]
  38. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  39. Suh, S. O., Marshall, C. J., McHugh, J. V. & Blackwell, M.(2003). Wood ingestion by passalid beetles in the presence of xylose-fermenting gut yeasts. Mol Ecol 12, 3137–3145.[CrossRef] [Google Scholar]
  40. Takeda, M., Suzuki, I. & Koizumi, J.(2005).Paenibacillus hodogayensis sp. nov., capable of degrading the polysaccharide produced by Sphaerotilus natans. Int J Syst Evol Microbiol 55, 737–741.[CrossRef] [Google Scholar]
  41. Tamaoka, J. & Komagata, K.(1984). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef] [Google Scholar]
  42. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  43. Velázquez, E., de Miguel, T., Poza, M., Rivas, R., Rosselló-Mora, R. & Villa, T. G.(2004).Paenibacillus favisporus sp. nov., a xylanolytic bacterium isolated from cow faeces. Int J Syst Evol Microbiol 54, 59–64.[CrossRef] [Google Scholar]
  44. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  45. Willcox, M. D. P.(1996). Identification and classification of species within the Streptococcus sanguis group. Aust Dent J 41, 107–112.[CrossRef] [Google Scholar]
  46. Yoon, J.-H., Lee, S. T. & Park, Y.-H.(1998). Inter- and intraspecific phylogenetic analysis of the genus Nocardioides and related taxa based on 16S rDNA sequences. Int J Syst Bacteriol 48, 187–194.[CrossRef] [Google Scholar]
  47. Yoon, J.-H., Kang, S.-J., Yeo, S.-H. & Oh, T.-K.(2005).Paenibacillus alkaliterrae sp. nov., isolated from an alkaline soil in Korea. Int J Syst Evol Microbiol 55, 2339–2344.[CrossRef] [Google Scholar]
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Paenibacillus pectinilyticus sp. nov., isolated from the gut of Diestrammena apicalis
Int J Syst Evol Microbiol 59, 1342 (2009); https://doi.org/10.1099/ijs.0.002261-0
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Transmission electron micrographs of cells of strain RCB-08 ( sp. nov.). [PDF](285 KB)

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Maximum-likelihood tree based on 16S rRNA gene sequences. [PDF](17 KB)

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Extended neighbour-joining tree based on 16S rRNA gene sequences. [PDF](28 KB)

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