1887

Abstract

During a search for exo-enzyme-producing bacteria in the gut of an insect, , a novel bacterium capable of degrading pectin was isolated. The isolate, designated strain RCB-08, comprised Gram-positive, endospore-forming, motile rods capable of growth at 15–30 °C and pH 6.0–8.7. The DNA G+C content of the isolate was 51.5 mol% and the predominant cellular fatty acid was anteiso-C (74.1 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RCB-08 was affiliated with a cluster within the , and was related most closely to NBRC 15376, with a sequence similarity of 96.7 %. The DNA–DNA relatedness value for strain RCB-08 with NBRC 15376 was 15.0 %. Strain RCB-08 hydrolysed pectin, but not cellulose, casein, starch or xylan. Strain RCB-08 could be clearly distinguished from other species on the basis of characteristics observed using a polyphasic approach. Therefore strain RCB-08 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RCB-08 (=KCTC 13222=CECT 7358).

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2009-06-01
2019-12-05
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Transmission electron micrographs of cells of strain RCB-08 ( sp. nov.). [PDF](285 KB)

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Maximum-likelihood tree based on 16S rRNA gene sequences. [PDF](17 KB)

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Extended neighbour-joining tree based on 16S rRNA gene sequences. [PDF](28 KB)

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