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A strictly aerobic, motile, short-rod-shaped, Gram-positive-staining actinomycete strain, designated MSL-01T, was isolated from a sediment sample from Bigeum Island of Korea. 16S rRNA gene sequence analysis revealed that the isolate MSL-01T belonged to the genus Nocardioides, with the highest sequence similarity to Nocardioides terrigena DS-17T (98.54 %), but the DNA–DNA relatedness to this type strain was 34 %. The physiological properties of strain MSL-01T differ from those of Nocardioides terrigena DS-17T and other species of Nocardioides. The diamino acid in the cell-wall peptidoglycan of strain MSL-01T is ll-diaminopimelic acid, the major menaquinone is MK-8(H4) and iso-C16 : 0 is the major fatty acid component. The name Nocardioides sediminis sp. nov. is proposed for the novel species, with the type strain MSL-01T (=DSM 19263T =KCTC 19271T).
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