A novel actinomycete, designated YIM 45751T, was isolated from a soil sample collected from the Xishuang Banna tropical rainforest, Yunnan province, south-west China, and was subjected to a polyphasic taxonomic study. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. Whole-cell hydrolysates contained ribose, xylose and glucose. The predominant menaquinones were MK-10(H4), MK-10(H6), MK-11(H4) and MK-11(H6). The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and three unknown phospholipids. 16S rRNA gene sequence analysis showed that Stackebrandtia nassauensis DSM 44728T was the closest phylogenetic neighbour (95.8 % 16S rRNA gene sequence similarity). On the basis of phylogenetic and some common chemotaxonomic data, the isolate should belong to the genus Stackebrandtia. However, phylogenetic analysis and comparison of physiological and chemotaxonomic characteristics demonstrated that the isolate is distinct from S. nassauensis DSM 44728T. Strain YIM 45751T represents a separate species of the genus Stackebrandtia, for which the name Stackebrandtia albiflava sp. nov. is proposed. The type strain is YIM 45751T (=DSM 45044T=CCTCC AA 206003T). Furthermore, the description of the genus Stackebrandtia is also required to be emended.
Collins, M. D. & Jones, D.(1980). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Microbiol48, 459–470.
[Google Scholar]
Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol100, 221–230.[CrossRef][Google Scholar]
Cui, X. L., Mao, P. H., Zeng, M., Li, W. J., Zhang, L. P., Xu, L. H. & Jiang, C. L.(2001).Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol51, 357–363.
[Google Scholar]
Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution39, 783–791.[CrossRef][Google Scholar]
Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol24, 54–63.[CrossRef][Google Scholar]
Kelly, K. L.(1964).ISCC-NBS Color-Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol16, 111–120.[CrossRef][Google Scholar]
Kimura, M.(1983).TheNeutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
Kovacs, N.(1956). Identification of Pseudomonas pyocyanea by oxidase reaction. Nature178, 703–704.
[Google Scholar]
Kroppenstedt, R. M.(1985).Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics17, 1244–1245.[CrossRef][Google Scholar]
Labeda, D. P. & Kroppenstedt, R. M.(2004). Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp. nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov. Int J Syst Evol Microbiol54, 2343–2346.[CrossRef][Google Scholar]
Labeda, D. P. & Kroppenstedt, R. M.(2005).Stackebrandtia nassauensis gen. nov., sp. nov. and emended description of the family Glycomycetaceae. Int J Syst Evol Microbiol55, 1687–1691.[CrossRef][Google Scholar]
Labeda, D. P., Testa, R. T., Lechevalier, M. P. & Lechevalier, H. A.(1985).Glycomyces, a new genus of the Actinomycetales. Int J Syst Bacteriol35, 417–421.[CrossRef][Google Scholar]
MacFaddin, J. F.(1980).Biochemical Tests for Identification of Medical Bacteria, 2nd edn. Baltimore, MD: Williams & Wilkins.
Meier, A., Kirschner, P., Schröder, K.-H., Wolters, J., Kroppenstedt, R. M. & Böttger, E. C.(1993).Mycobacterium intermedium sp. nov. Int J Syst Bacteriol43, 204–209.[CrossRef][Google Scholar]
Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol39, 159–167.[CrossRef][Google Scholar]
Minnikin, D. E., Collins, M. D. & Goodfellow, M.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Microbiol47, 87–95.
[Google Scholar]
Pridham, T. G. & Lyons, A. J.(1980). Methodologies for Actinomycetales with special reference to streptomycetes and streptoverticillia. In Actinomycete Taxonomy, pp. 153–224. Edited by A. Dietz & D. W. Thayer. Special publication no. 6. Arlington, VA: Society for Industrial Microbiology.
Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4, 406–425.
[Google Scholar]
Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol16, 313–340.[CrossRef][Google Scholar]
Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol44, 846–849.[CrossRef][Google Scholar]
Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L.(1997). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol47, 479–491.[CrossRef][Google Scholar]
Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol28, 226–231.
[Google Scholar]
Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H.(1983). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Microbiol54, 31–36.
[Google Scholar]
Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res25, 4876–4882.[CrossRef][Google Scholar]
Vickers, J. C., Williams, S. T. & Ross, G. W.(1984). A taxonomic approach to selective isolation of streptomycetes from soil. In Biological, Biochemical and Biomedical Aspects of Actinomycetes, pp. 553–561. Edited by L. Ortiz-Ortiz, L. F. Bojalil & V. Yakoleff. London: Academic Press.
International Journal of Systematic and Evolutionary
Microbiology vol.
59 , part 3, pp. 574 – 577
Supplementary Fig. S1. Scanning electron micrograph of
strain YIM 45751
T
Supplementary Fig. S2. Phylogenetic tree showing the
relationship between strain YIM 45751
Tand representatives of the family
Glycomycetaceae , based on 16S rRNA gene sequences