A novel actinomycete, designated YIM 45751, was isolated from a soil sample collected from the Xishuang Banna tropical rainforest, Yunnan province, south-west China, and was subjected to a polyphasic taxonomic study. The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. Whole-cell hydrolysates contained ribose, xylose and glucose. The predominant menaquinones were MK-10(H), MK-10(H), MK-11(H) and MK-11(H). The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and three unknown phospholipids. 16S rRNA gene sequence analysis showed that DSM 44728 was the closest phylogenetic neighbour (95.8 % 16S rRNA gene sequence similarity). On the basis of phylogenetic and some common chemotaxonomic data, the isolate should belong to the genus . However, phylogenetic analysis and comparison of physiological and chemotaxonomic characteristics demonstrated that the isolate is distinct from DSM 44728. Strain YIM 45751 represents a separate species of the genus , for which the name sp. nov. is proposed. The type strain is YIM 45751 (=DSM 45044=CCTCC AA 206003). Furthermore, the description of the genus is also required to be emended.


Article metrics loading...

Loading full text...

Full text loading...



  1. Collins, M. D. & Jones, D.(1980). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Microbiol 48, 459–470. [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  3. Cui, X. L., Mao, P. H., Zeng, M., Li, W. J., Zhang, L. P., Xu, L. H. & Jiang, C. L.(2001).Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363. [Google Scholar]
  4. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  5. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef] [Google Scholar]
  6. Kelly, K. L.(1964).ISCC-NBS Color-Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  7. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  8. Kimura, M.(1983).TheNeutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  9. Kovacs, N.(1956). Identification of Pseudomonas pyocyanea by oxidase reaction. Nature 178, 703–704. [Google Scholar]
  10. Kroppenstedt, R. M.(1985).Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
  11. Kumar, S., Tamura, K., Jakobsen, I. B. & Nei, M.(2001).mega2: molecular evolutionary genetics analysis software. Bioinformatics 17, 1244–1245.[CrossRef] [Google Scholar]
  12. Labeda, D. P. & Kroppenstedt, R. M.(2004). Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp. nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov. Int J Syst Evol Microbiol 54, 2343–2346.[CrossRef] [Google Scholar]
  13. Labeda, D. P. & Kroppenstedt, R. M.(2005).Stackebrandtia nassauensis gen. nov., sp. nov. and emended description of the family Glycomycetaceae. Int J Syst Evol Microbiol 55, 1687–1691.[CrossRef] [Google Scholar]
  14. Labeda, D. P., Testa, R. T., Lechevalier, M. P. & Lechevalier, H. A.(1985).Glycomyces, a new genus of the Actinomycetales. Int J Syst Bacteriol 35, 417–421.[CrossRef] [Google Scholar]
  15. MacFaddin, J. F.(1980).Biochemical Tests for Identification of Medical Bacteria, 2nd edn. Baltimore, MD: Williams & Wilkins.
  16. Meier, A., Kirschner, P., Schröder, K.-H., Wolters, J., Kroppenstedt, R. M. & Böttger, E. C.(1993).Mycobacterium intermedium sp. nov. Int J Syst Bacteriol 43, 204–209.[CrossRef] [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  18. Minnikin, D. E., Collins, M. D. & Goodfellow, M.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Microbiol 47, 87–95. [Google Scholar]
  19. Pridham, T. G. & Lyons, A. J.(1980). Methodologies for Actinomycetales with special reference to streptomycetes and streptoverticillia. In Actinomycete Taxonomy, pp. 153–224. Edited by A. Dietz & D. W. Thayer. Special publication no. 6. Arlington, VA: Society for Industrial Microbiology.
  20. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  21. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  22. Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef] [Google Scholar]
  23. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L.(1997). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef] [Google Scholar]
  24. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  25. Tamaoka, J., Katayama-Fujimura, Y. & Kuraishi, H.(1983). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Microbiol 54, 31–36. [Google Scholar]
  26. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  27. Vickers, J. C., Williams, S. T. & Ross, G. W.(1984). A taxonomic approach to selective isolation of streptomycetes from soil. In Biological, Biochemical and Biomedical Aspects of Actinomycetes, pp. 553–561. Edited by L. Ortiz-Ortiz, L. F. Bojalil & V. Yakoleff. London: Academic Press.

Data & Media loading...


vol. , part 3, pp. 574 – 577

Scanning electron micrograph of strain YIM 45751

Phylogenetic tree showing the relationship between strain YIM 45751 and representatives of the family , based on 16S rRNA gene sequences

[ Single PDF file] (357 KB)


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error