The taxonomic position of an actinomycete, designated SCSIO 00231, isolated from a sediment sample collected from the northern South China Sea, was determined by using a polyphasic approach. The organism formed fragmented substrate hyphae and sparse aerial mycelium on modified ISP 2 medium. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain SCSIO 00231 fell into the family , in which it formed a distinct lineage and was loosely associated with DSM 44069, with 93 % similarity. The other closest phylogenetic neighbours were NRRL 2338 (92.6 % similarity) and DSM 44468 (93.1 % similarity). The isolate had cell-wall type IV (-diaminopimelic acid and whole-cell sugars arabinose, galactose and glucose) and phospholipid type III. The predominant menaquinone was MK-9(H). The G+C content of the genomic DNA was 69 mol%. Based on these data, strain SCSIO 00231 can be readily distinguished from previously described organisms and represents a new genus within the family . The name gen. nov. is proposed, with the novel species sp. nov. The type strain of is SCSIO 00231 (=KCTC 19433 =CCTCC AA208009).


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Scanning electron micrographs showing fragmented substrate mycelium and sparse aerial mycelium of gen. nov., sp. nov. SCSIO 00231 after incubation on modified ISP 2 medium for 4 weeks at 28 °C.


Scanning electron micrographs showing fragmented substrate mycelium and sparse aerial mycelium of gen. nov., sp. nov. SCSIO 00231 after incubation on modified ISP 2 medium for 4 weeks at 28 °C.

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