1887

Abstract

Two vancomycin-resistant, strictly anaerobic, Gram-positive, rod-shaped, spore-forming organisms (strains CCRI-9842 and CCRI-9929) isolated from human faecal specimens in Québec, Canada, and Australia were characterized using phenotypic, biochemical and molecular taxonomic methods. Pairwise analysis of the 16S rRNA gene sequences showed that both strains were closely related to each other genetically (displaying 99.2 % sequence similarity) and represented a previously unknown subline within the rRNA group of organisms (rRNA cluster XIVa of the genus ). Strains CCRI-9842 and CCRI-9929 used carbohydrates as fermentable substrates, producing acetic acid as the major product of glucose metabolism. The novel strains were most closely related to , and , but morphological, biochemical and phylogenetic studies demonstrated that they represent a previously unidentified species of the genus . This was confirmed by the unique cellular fatty acid composition of strains CCRI-9842 and CCRI-9929. Therefore, on the basis of data from the polyphasic taxonomic analysis, it is proposed that strains CCRI-9842 and CCRI-9929 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CCRI-9842 (=CCUG 54291=JCM 14986=NML 03-A-015).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.001958-0
2009-03-01
2019-12-08
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/3/498.html?itemId=/content/journal/ijsem/10.1099/ijs.0.001958-0&mimeType=html&fmt=ahah

References

  1. Backhed, F., Ley, R. E., Sonnenburg, J. L., Peterson, D. A. & Gordon, J. I. ( 2005; ). Host-bacterial mutualism in the human intestine. Science 307, 1915–1920.[CrossRef]
    [Google Scholar]
  2. Ballard, S. A., Pertile, K. K., Lim, M., Johnson, P. D. & Grayson, M. L. ( 2005; ). Molecular characterization of vanB elements in naturally occurring gut anaerobes. Antimicrob Agents Chemother 49, 1688–1694.[CrossRef]
    [Google Scholar]
  3. Bernard, K. A., Shuttleworth, L., Munro, C., Forbes-Faulkner, J. C., Pitt, D., Norton, J. H. & Thomas, A. D. ( 2002; ). Propionibacterium australiense sp. nov. derived from granulomatous bovine lesions. Anaerobe 8, 41–47.[CrossRef]
    [Google Scholar]
  4. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. ( 1994; ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812–826.[CrossRef]
    [Google Scholar]
  5. Domingo, M. C., Huletsky, A., Bernal, A., Giroux, R., Boudreau, D. K., Picard, F. J. & Bergeron, M. G. ( 2005; ). Characterization of a Tn5382-like transposon containing the vanB2 gene cluster in a Clostridium strain isolated from human faeces. J Antimicrob Chemother 55, 466–474.[CrossRef]
    [Google Scholar]
  6. Eckburg, P. B., Bik, E. M., Bernstein, C. N., Purdom, E., Dethlefsen, L., Sargent, M., Gill, S. R., Nelson, K. E. & Relman, D. A. ( 2005; ). Diversity of the human intestinal microbial flora. Science 308, 1635–1638.[CrossRef]
    [Google Scholar]
  7. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  8. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). In Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  9. Hooper, L. V., Midtvedt, T. & Gordon, J. I. ( 2002; ). How host-microbial interactions shape the nutrient environment of the mammalian intestine. Annu Rev Nutr 22, 283–307.[CrossRef]
    [Google Scholar]
  10. Jousimies-Somer, H. R., Summanen, P., Citron, D. M., Baron, E. J., Wexler, H. M. & Finegold, S. M. ( 2002; ). In Wadsworth Anaerobic Bacteriology Manual, 6th edn. Belmont, CA: Star Publishing.
  11. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  12. Mohan, R., Namsolleck, P., Lawson, P. A., Osterhoff, M., Collins, M. D., Alpert, C. A. & Blaut, M. ( 2006; ). Clostridium asparagiforme sp. nov., isolated from a human faecal sample. Syst Appl Microbiol 29, 292–299.[CrossRef]
    [Google Scholar]
  13. NCCLS ( 2004; ). Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria, 6th edn. Approved Standard M11–A6; 24. Wayne, PA: National Committee for Clinical Laboratory Standards.
  14. Paradis, S., Boissinot, M., Paquette, N., Bélanger, S. D., Martel, E. A., Boudreau, D. K., Picard, F. J., Ouellette, M., Roy, P. H. & other authors ( 2005; ). Phylogeny of the Enterobacteriaceae based on genes encoding elongation factor Tu and F-ATPase β-subunit. Int J Syst Evol Microbiol 55, 2013–2025.[CrossRef]
    [Google Scholar]
  15. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  16. Song, Y., Liu, C., Molitoris, D. R., Tomzynski, T. J., Lawson, P. A., Collins, M. D. & Finegold, S. M. ( 2003; ). Clostridium bolteae sp. nov., isolated from human sources. Syst Appl Microbiol 26, 84–89.[CrossRef]
    [Google Scholar]
  17. Stackebrandt, E. & Ebers, J. ( 2006; ). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155.
    [Google Scholar]
  18. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  19. Stinear, T. P., Olden, D. C., Johnson, P. D., Davies, J. K. & Grayson, M. L. ( 2001; ). Enterococcal vanB resistance locus in anaerobic bacteria in human faeces. Lancet 357, 855–856.[CrossRef]
    [Google Scholar]
  20. Suau, A., Bonnet, R., Sutren, M., Godon, J. J., Gibson, G. R., Collins, M. D. & Dore, J. ( 1999; ). Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 65, 4799–4807.
    [Google Scholar]
  21. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  22. Zoetendal, E. G., Vaughan, E. E. & de Vos, W. M. ( 2006; ). A microbial world within us. Mol Microbiol 59, 1639–1650.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.001958-0
Loading
/content/journal/ijsem/10.1099/ijs.0.001958-0
Loading

Data & Media loading...

Supplements

vol. , part 3, pp. 498 - 503

Neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences of sp. nov. and Gram-positive bacteria (87 taxa) belonging to clusters XIVa ( group) and XIVb of the subphylum.

Maximum-parsimony cladogram, based on 16S rRNA gene sequences of and Gram- positive bacteria (87 taxa) belonging to clusters XIVa ( group) and XIVb of the subphylum.

Maximum-parsimony cladograms, based on 16S rRNA gene sequences, showing the relationships of and related taxa within cluster XIVa of the genus .

Maximum-likelihood cladogram, based on 16S rRNA gene sequences, showing the relationships of and related taxa within cluster XIVa of the genus .

Combined File [ PDF] 458 KB



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error