Two vancomycin-resistant, strictly anaerobic, Gram-positive, rod-shaped, spore-forming organisms (strains CCRI-9842 and CCRI-9929) isolated from human faecal specimens in Québec, Canada, and Australia were characterized using phenotypic, biochemical and molecular taxonomic methods. Pairwise analysis of the 16S rRNA gene sequences showed that both strains were closely related to each other genetically (displaying 99.2 % sequence similarity) and represented a previously unknown subline within the rRNA group of organisms (rRNA cluster XIVa of the genus ). Strains CCRI-9842 and CCRI-9929 used carbohydrates as fermentable substrates, producing acetic acid as the major product of glucose metabolism. The novel strains were most closely related to , and , but morphological, biochemical and phylogenetic studies demonstrated that they represent a previously unidentified species of the genus . This was confirmed by the unique cellular fatty acid composition of strains CCRI-9842 and CCRI-9929. Therefore, on the basis of data from the polyphasic taxonomic analysis, it is proposed that strains CCRI-9842 and CCRI-9929 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CCRI-9842 (=CCUG 54291=JCM 14986=NML 03-A-015).


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vol. , part 3, pp. 498 - 503

Neighbour-joining phylogenetic tree, based on 16S rRNA gene sequences of sp. nov. and Gram-positive bacteria (87 taxa) belonging to clusters XIVa ( group) and XIVb of the subphylum.

Maximum-parsimony cladogram, based on 16S rRNA gene sequences of and Gram- positive bacteria (87 taxa) belonging to clusters XIVa ( group) and XIVb of the subphylum.

Maximum-parsimony cladograms, based on 16S rRNA gene sequences, showing the relationships of and related taxa within cluster XIVa of the genus .

Maximum-likelihood cladogram, based on 16S rRNA gene sequences, showing the relationships of and related taxa within cluster XIVa of the genus .

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