RT Journal Article SR Electronic(1) A1 Lai, Qiliang A1 Yuan, Jun A1 Wang, Baojiang A1 Sun, Fengqin A1 Qiao, Nan A1 Zheng, Tianling A1 Shao, ZongzeYR 2009 T1 Bowmanella pacifica sp. nov., isolated from a pyrene-degrading consortium JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 7 SP 1579 OP 1582 DO https://doi.org/10.1099/ijs.0.001826-0 PB Microbiology Society, SN 1466-5034, AB A taxonomic study was carried out on a strain, designated W3-3AT, which was isolated from a pyrene-degrading consortium, enriched from sediment of the Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain W3-3AT belonged to the genus Bowmanella, with the highest sequence similarity (99.0 %) with Bowmanella denitrificans BD1T, whereas sequence similarities with other species were less than 93 %. The nucleotide sequence similarity of both gyrB and rpoD genes of strain W3-3AT and B. denitrificans BD1T was 81.1 %. However, the protein sequence similarities of the gyrB and rpoD genes of strain W3-3AT and B. denitrificans BD1T were 96.1 % and 91.0 %, respectively. Phylogenetic trees based on these housekeeping genes showed that strain W3-3AT and B. denitrificans BD1T formed a distinct lineage in the Gammaproteobacteria. The DNA–DNA hybridization value between strain W3-3AT and B. denitrificans BD1T was 43 %. Strain W3-3AT could also be differentiated from B. denitrificans BD1T based on the repetitive extragenic palindromic DNA-PCR fingerprint. The G+C content of the chromosomal DNA of strain W3-3AT was 49 mol%. The combined genotypic and phenotypic data showed that strain W3-3AT represents a novel species of the genus Bowmanella, for which the name Bowmanella pacifica sp. nov. is proposed, with the type strain W3-3AT (=CGMCC 1.7086T=LMG 24568T=MCCC 1A01018T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.001826-0