1887

Abstract

A taxonomic study was carried out on a strain, designated W3-3A, which was isolated from a pyrene-degrading consortium, enriched from sediment of the Pacific Ocean. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain W3-3A belonged to the genus , with the highest sequence similarity (99.0 %) with BD1, whereas sequence similarities with other species were less than 93 %. The nucleotide sequence similarity of both and genes of strain W3-3A and BD1 was 81.1 %. However, the protein sequence similarities of the and genes of strain W3-3A and BD1 were 96.1 % and 91.0 %, respectively. Phylogenetic trees based on these housekeeping genes showed that strain W3-3A and BD1 formed a distinct lineage in the . The DNA–DNA hybridization value between strain W3-3A and BD1 was 43 %. Strain W3-3A could also be differentiated from BD1 based on the repetitive extragenic palindromic DNA-PCR fingerprint. The G+C content of the chromosomal DNA of strain W3-3A was 49 mol%. The combined genotypic and phenotypic data showed that strain W3-3A represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain W3-3A (=CGMCC 1.7086=LMG 24568=MCCC 1A01018).

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2009-07-01
2020-01-19
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Cellular fatty acid contents of strain W3-3A and . [PDF](46 KB)

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Neighbour-joining tree showing the position of strain W3-3A based on gene sequences. [PDF](15 KB)

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Neighbour-joining tree showing the position of strain W3-3A based on gene sequences. [PDF](15 KB)

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rep-PCR comparison of strain W3-3A and . [PDF](35 KB)

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Transmission electron micrographs of strain W3-3A . [PDF](131 KB)

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Polar lipids of strain W3-3A and . [PDF](31 KB)

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