A strictly anaerobic, mesophilic, cellulolytic bacterial strain, designated CDT-1, was isolated from rice-straw residue from a methanogenic reactor treating waste from cattle farms. The isolation was performed using enrichment culture with filter paper as a substrate. Cells stained Gram-negative, but reacted Gram-positively in the KOH test. Cells were slightly curved rods and were motile by means of peritrichous flagella. The strain produced yellow pigment when grown on filter-paper fragments. Although spore formation was not confirmed microscopically, thermotolerant cells were produced when the strain was grown on filter paper. The optimum temperature for growth was 33 °C and the optimum pH was 7.4. Oxidase, catalase and nitrate-reducing activities were absent. The strain utilized xylose, fructose, glucose, cellobiose, xylooligosaccharide, cellulose (filter-paper fragments and ball-milled filter paper) and xylan. The major fermentation products were acetate, ethanol, H and CO. The major cellular fatty acids were iso-C, iso-C and C DMA. The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 40.7 mol%. On the basis of 16S rRNA gene sequence similarities, strain CDT-1 could be placed in cluster III of the genus , being closely related to type strains of (96.6 % sequence similarity), (96.2 %) and (96.1 %). On the basis of the cellular, physiological and phylogenetic differences between CDT-1 and its close relatives, this strain represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CDT-1 (=JCM 14807=DSM 19573).


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