1887

Abstract

A strictly anaerobic, mesophilic, cellulolytic bacterial strain, designated CDT-1, was isolated from rice-straw residue from a methanogenic reactor treating waste from cattle farms. The isolation was performed using enrichment culture with filter paper as a substrate. Cells stained Gram-negative, but reacted Gram-positively in the KOH test. Cells were slightly curved rods and were motile by means of peritrichous flagella. The strain produced yellow pigment when grown on filter-paper fragments. Although spore formation was not confirmed microscopically, thermotolerant cells were produced when the strain was grown on filter paper. The optimum temperature for growth was 33 °C and the optimum pH was 7.4. Oxidase, catalase and nitrate-reducing activities were absent. The strain utilized xylose, fructose, glucose, cellobiose, xylooligosaccharide, cellulose (filter-paper fragments and ball-milled filter paper) and xylan. The major fermentation products were acetate, ethanol, H and CO. The major cellular fatty acids were iso-C, iso-C and C DMA. The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The genomic DNA G+C content was 40.7 mol%. On the basis of 16S rRNA gene sequence similarities, strain CDT-1 could be placed in cluster III of the genus , being closely related to type strains of (96.6 % sequence similarity), (96.2 %) and (96.1 %). On the basis of the cellular, physiological and phylogenetic differences between CDT-1 and its close relatives, this strain represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CDT-1 (=JCM 14807=DSM 19573).

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2009-05-01
2019-10-16
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References

  1. Akasaka, H., Izawa, T., Ueki, K. & Ueki, A. ( 2003a; ). Phylogeny of numerically abundant culturable anaerobic bacteria associated with degradation of rice plant residue in Japanese paddy field soil. FEMS Microbiol Ecol 43, 149–161.[CrossRef]
    [Google Scholar]
  2. Akasaka, H., Ueki, A., Hanada, S., Kamagata, Y. & Ueki, K. ( 2003b; ). Propionicimonas paludicola gen. nov., sp. nov., a novel facultatively anaerobic, Gram-positive, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil. Int J Syst Evol Microbiol 53, 1991–1998.[CrossRef]
    [Google Scholar]
  3. Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W. & Lipman, D. J. ( 1997; ). Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25, 3389–3402.[CrossRef]
    [Google Scholar]
  4. Blenden, D. C. & Goldberg, H. S. ( 1965; ). Silver impregnation stain for Leptospira and flagella. J Bacteriol 89, 899–900.
    [Google Scholar]
  5. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. E. ( 1994; ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812–826.[CrossRef]
    [Google Scholar]
  6. Hethener, P., Brauman, A. & Garcia, J. L. ( 1992; ). Clostridium termitidis sp. nov., a cellulolytic bacterium from the gut of the wood-feeding termite, Nasutitermes lujae. Syst Appl Microbiol 15, 52–58.[CrossRef]
    [Google Scholar]
  7. Holdeman, L. V., Cato, E. P. & Moore, W. E. C. ( 1977; ). Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
  8. Hungate, R. E. ( 1966; ). The Rumen and Its Microbes. New York: Academic Press.
  9. Kaku, N., Ueki, A., Fujii, H. & Ueki, K. ( 2000; ). Methanogenic activities on rice roots and plant residue and their contributions to methanogenesis in wetland rice field soil. Soil Biol Biochem 32, 2001–2010.[CrossRef]
    [Google Scholar]
  10. Kato, S., Haruta, S., Cui, Z. J., Ishii, M., Yokota, A. & Igarashi, Y. ( 2004; ). Clostridium straminisolvens sp. nov., a moderately thermophilic, aerotolerant and cellulolytic bacterium isolated from a cellulose-degrading bacterial community. Int J Syst Evol Microbiol 54, 2043–2047.[CrossRef]
    [Google Scholar]
  11. Komagata, K. & Suzuki, K. ( 1987; ). Lipid and cell-wall analysis in bacterial systematics. Methods Microbiol 19, 161–207.
    [Google Scholar]
  12. Kopečný, J. & Hodrová, B. ( 1997; ). The effect of yellow affinity substance on cellulases of Ruminococcus flavefaciens. Lett Appl Microbiol 25, 191–196.[CrossRef]
    [Google Scholar]
  13. Ljungdahl, L. G., Pettersson, B., Eriksson, K. E. & Wiegel, J. ( 1983; ). A yellow affinity substance involved in the cellulolytic system of Clostridium thermocellum. Curr Microbiol 9, 195–200.[CrossRef]
    [Google Scholar]
  14. Madden, R. H., Bryder, M. J. & Poole, N. J. ( 1982; ). Isolation and characterization of an anaerobic, cellulolytic bacterium, Clostridium papyrosolvens sp. nov. Int J Syst Bacteriol 32, 87–91.[CrossRef]
    [Google Scholar]
  15. Miller, L. T. ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxyl acids. J Clin Microbiol 16, 584–586.
    [Google Scholar]
  16. Miyagawa, E., Azuma, R. & Suto, E. ( 1979; ). Cellular fatty acid composition in Gram-negative obligately anaerobic rods. J Gen Appl Microbiol 25, 41–51.[CrossRef]
    [Google Scholar]
  17. Monserrate, E., Leschine, S. B. & Canale-Parola, E. ( 2001; ). Clostridium hungatei sp. nov., a mesophilic, N2-fixing cellulolytic bacterium isolated from soil. Int J Syst Evol Microbiol 51, 123–132.
    [Google Scholar]
  18. Moore, L. V. H., Bourne, D. M. & Moore, W. E. C. ( 1994; ). Comparative distribution and taxonomic value of cellular fatty acids in thirty-three genera of anaerobic Gram-negative bacilli. Int J Syst Bacteriol 44, 338–347.[CrossRef]
    [Google Scholar]
  19. Petitdemange, E., Caillet, F., Giallo, J. & Gaudin, C. ( 1984; ). Clostridium cellulolyticum sp. nov., a cellulolytic, mesophilic species from decayed grass. Int J Syst Bacteriol 34, 155–159.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  22. Sukhumavasi, J., Ohmiya, K., Shimizu, S. & Ueno, K. ( 1988; ). Clostridium josui sp. nov., a cellulolytic, moderate thermophilic species from Thai compost. Int J Syst Bacteriol 38, 179–182.[CrossRef]
    [Google Scholar]
  23. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  24. Ueki, A. & Suto, T. ( 1979; ). Cellular fatty acid composition of sulfate-reducing bacteria. J Gen Appl Microbiol 25, 185–196.[CrossRef]
    [Google Scholar]
  25. Ueki, A., Matsuda, K. & Ohtsuki, C. ( 1986; ). Sulfate reduction in the anaerobic digestion of animal waste. J Gen Appl Microbiol 32, 111–123.[CrossRef]
    [Google Scholar]
  26. Ueki, A., Akasaka, H., Suzuki, D. & Ueki, K. ( 2006a; ). Paludibacter propionicigenes gen. nov., sp. nov., a novel strictly anaerobic, Gram-negative, propionate-producing bacterium isolated from plant residue in irrigated rice-field soil in Japan. Int J Syst Evol Microbiol 56, 39–44.[CrossRef]
    [Google Scholar]
  27. Ueki, A., Akasaka, H., Suzuki, D., Hattori, S. & Ueki, K. ( 2006b; ). Xylanibacter oryzae gen. nov., sp. nov., a novel strictly anaerobic, Gram-negative, xylanolytic bacterium isolated from rice-plant residue in flooded rice-field soil in Japan. Int J Syst Evol Microbiol 56, 2215–2221.[CrossRef]
    [Google Scholar]
  28. Ueki, A., Akasaka, H., Suzuki, D., Satoh, A. & Ueki, K. ( 2007; ). Prevotella paludivivens sp. nov., a novel strictly anaerobic, Gram-negative, hemicellulose-decomposing bacterium isolated from plant residue and rice roots in irrigated rice-field soil. Int J Syst Evol Microbiol 57, 1803–1809.[CrossRef]
    [Google Scholar]
  29. Wallace, W. H. & Gates, J. E. ( 1986; ). Identification of Eubacteria isolated from leaf cavities of four species of the N-fixing Azolla fern as Arthrobacter Conn and Dimmick. Appl Environ Microbiol 52, 425–429.
    [Google Scholar]
  30. Willems, A. & Collins, M. D. ( 1995; ). Phylogenetic analysis of Ruminococcus flavefaciens, the type species of the genus Ruminococcus, does not support the reclassification of Streptococcus hansenii and Peptostreptococcus productus as ruminococci. Int J Syst Bacteriol 45, 572–575.[CrossRef]
    [Google Scholar]
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