A taxonomic study was performed on two isolates, strains RF3 and RF21, recovered from a thiosulfate-oxidizing enrichment culture. Comparative 16S rRNA gene sequence analysis showed that these isolates were clearly affiliated with the class . Strains RF3 and RF21 were most closely related to NA10B (97.9 and 98.2 % 16S rRNA gene sequence similarity, respectively). The level of 16S rRNA gene sequence similarity between strains RF3 and RF21 was 99.8 %. The two isolates were Gram-negative, aerobic and denitrifying. Growth was observed at 7–35 °C. Physiological and biochemical properties of strains RF3 and RF21 were different from those of NA10B in that strains RF3 and RF21 were able to utilize -histidine, 2-ketogluconate and caprate, but unable to utilize suberate. The G+C contents of the genomic DNA of strains RF3 and RF21 were 62.9 and 62.8 mol%, respectively, and the predominant quinone was ubiquinone-8. Strains RF3 and RF21 possessed C, C cyclo, and C 7 and/or iso-C 2-OH as major fatty acids. DNA–DNA relatedness data indicated that strains RF3 and RF21 represent a genomic species separate from . On the basis of the evidence presented from this polyphasic analysis, it is suggested that strains RF3 and RF21 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RF3 (=KCTC 22225=LMG 24467).


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vol. , part 2, pp. 218 - 221

Phylogenetic relationships of isolates RF3 and RF21 and some related species of the genera , , , , and on the basis of 16S rRNA gene sequences. [ PDF] 47 KB

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