@article{mbs:/content/journal/ijsem/10.1099/ijs.0.001552-0, author = "Wu, Yehui and Lai, Qiliang and Zhou, Zhongwen and Qiao, Nan and Liu, Chenli and Shao, Zongze", title = "Alcanivorax hongdengensis sp. nov., an alkane-degrading bacterium isolated from surface seawater of the straits of Malacca and Singapore, producing a lipopeptide as its biosurfactant", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2009", volume = "59", number = "6", pages = "1474-1479", doi = "https://doi.org/10.1099/ijs.0.001552-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.001552-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "ITS, internal transcribed spacer", abstract = "A taxonomic study was carried out on strain A-11-3T, which was isolated from an oil-enriched consortia from the surface seawater of Hong-Deng dock in the Straits of Malacca and Singapore. Cells were aerobic, Gram-negative, non-spore-forming irregular rods. The strain was catalase- and oxidase-negative. It grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. 16S rRNA gene sequence comparisons showed that strain A-11-3T was most closely related to the type strains of Alcanivorax jadensis (96.8 % sequence similarity), Alcanivorax borkumensis (96.8 %), Alcanivorax dieselolei (94.8 %), Alcanivorax venustensis (94.2 %) and Alcanivorax balearicus (94.0 %). The predominant fatty acids were C16 : 0 (31.2 %), C18 : 1 ω7c (24.8 %), C18 : 0 (9.6 %), C12 : 0 (8.3 %), C16 : 1 ω7c (8.3 %) and C16 : 0 3-OH (5.1 %). The G+C content of the genomic DNA was 54.7 mol%. Moreover, the strain produced lipopeptides as its surface-active compounds. According to physiological and biochemical tests, DNA–DNA hybridization results and sequence comparisons of the 16S–23S internal transcribed spacer, the gyrB gene and the alkane hydroxylase gene alkB1, strain A-11-3T was affiliated with the genus Alcanivorax but could be readily distinguished from recognized Alcanivorax species. Therefore strain A-11-3T represents a novel species of the genus Alcanivorax for which the name Alcanivorax hongdengensis sp. nov. is proposed. The type strain is A-11-3T (=CGMCC 1.7084T=LMG 24624T=MCCC 1A01496T).", }