A taxonomic study was carried out on strain A-11-3, which was isolated from an oil-enriched consortia from the surface seawater of Hong-Deng dock in the Straits of Malacca and Singapore. Cells were aerobic, Gram-negative, non-spore-forming irregular rods. The strain was catalase- and oxidase-negative. It grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. 16S rRNA gene sequence comparisons showed that strain A-11-3 was most closely related to the type strains of (96.8 % sequence similarity), (96.8 %), (94.8 %), (94.2 %) and (94.0 %). The predominant fatty acids were C (31.2 %), C 7 (24.8 %), C (9.6 %), C (8.3 %), C 7 (8.3 %) and C 3-OH (5.1 %). The G+C content of the genomic DNA was 54.7 mol%. Moreover, the strain produced lipopeptides as its surface-active compounds. According to physiological and biochemical tests, DNA–DNA hybridization results and sequence comparisons of the 16S–23S internal transcribed spacer, the gene and the alkane hydroxylase gene , strain A-11-3 was affiliated with the genus but could be readily distinguished from recognized species. Therefore strain A-11-3 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is A-11-3 (=CGMCC 1.7084=LMG 24624=MCCC 1A01496).


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Neighbour-joining tree based on 16S-23S intergenic sequences (ITS) of strain A-11-3 ( sp. nov.) and recognized species of the genus . [PDF](16 KB)


Neighbour-joining tree based on partial sequences of strain A-11-3 ( sp. nov.) and recognized species of the genus . [PDF](15 KB)


Neighbour-joining tree based on partial sequences of strain A-11-3 ( sp. nov.) and recognized species of the genus . [PDF](15 KB)


Transmission electron micrographs of negatively stained cells of strain A-11-3 ( sp. nov.). [PDF](146 KB)


Cellular fatty acid profiles of strain A-11-3 ( sp. nov.) and recognized species of the genus . [PDF](88 KB)


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