A strictly anaerobic Fe(III)-reducing bacterium, designated strain Red1, was isolated from the production water of the Redwash oilfield, USA. The cells were motile rods (1–5×0.5–0.6 μm) that stained Gram-negative and possessed polar flagella. Strain Red1 obtained energy from the reduction of Fe(III), Mn(IV), nitrate, elemental sulfur and trimethylamine -oxide in the presence of a wide range of electron donors, including a variety of organic acids, alcohols, biological extracts and hydrogen. Strain Red1 was incapable of fermentative growth. The novel isolate grew optimally at 40 °C (temperature range for growth, 30–50 °C) and at pH 7 (pH range, 6–9) with 2 % (w/v) NaCl (NaCl range, 0.1–10 %, w/v). The DNA G+C content was 52.5 mol%. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Red1 was a member of the order within the class and most closely related to Z-0531 (95.8 %), SDBY1 (92.5 %) and ‘ BB1 (92.4 %). On the basis of phenotypic and phylogenetic differences, the novel strain is proposed to represent a novel species, sp. nov. (type strain Red1=JCM 15104=KCTC 5626).


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