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Abstract

Eighteen isolates of a nonchromogenic, slowly growing, non-tuberculous species of the genus were cultured from respiratory specimens obtained over the last eight years from 17 patients in the Netherlands. These isolates were grouped because they revealed a unique 16S rRNA gene sequence and were related to . None of the 17 patients met the American Thoracic Society diagnostic criteria for non-tuberculous mycobacterial disease, which distinguishes the novel isolates from the related species, . A polyphasic taxonomic approach, including identification by biochemical and phenotypical analysis, gene sequencing and PCR restriction enzyme pattern analysis, and sequence analyses of the gene and 16S–23S internal transcribed spacer supported the separate species status of the novel isolates. The name sp. nov. is proposed for the novel strains. The type strain is NLA000500338 (=DSM 45145=CIP 109766). A more distinctive taxonomy of NTM is a prerequisite for the assessment of their clinical relevance.

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2009-04-01
2020-01-26
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References

  1. Centers for Disease Control and Prevention ( 1996; ). Standardized Method for HPLC Identification of Mycobacteria. Atlanta, US: Department of Health and Human Services, Public Health Service.
  2. Dauendorffer, J. N., Laurain, C., Weber, M. & Dailloux, M. ( 2002; ). In vitro sensitivity of Mycobacterium xenopi to five antibiotics. Pathol Biol 50, 591–594.[CrossRef]
    [Google Scholar]
  3. Griffith, D. E., Aksamit, T., Brown-Elliot, B. A., Catanzaro, A., Daley, C., Gordin, F., Holland, S. M., Horsburgh, R., Huitt, G. & other authors ( 2007; ). An official ATS/IDSA statement: diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases. Am J Respir Crit Care Med 175, 367–416.[CrossRef]
    [Google Scholar]
  4. Hall, L., Doerr, A., Wohlfiel, S. L. & Roberts, G. D. ( 2003; ). Evaluation of the MicroSeq system for identification of mycobacteria by 16S ribosomal DNA sequencing and its integration into a routine mycobacteriology laboratory. J Clin Microbiol 41, 1447–1453.[CrossRef]
    [Google Scholar]
  5. Henry, M. T., Inamdar, L., O'Riordain, D., Schweiger, M. & Watson, J. P. ( 2004; ). Nontuberculous mycobacteria in non-HIV patients: epidemiology, treatment and response. Eur Respir J 23, 741–746.[CrossRef]
    [Google Scholar]
  6. Kim, B. J., Lee, S. H., Lyu, M. A., Kim, S. J., Bai, G. H., Chae, G. T., Kim, E. C., Cha, C. Y. & Kook, Y. H. ( 1999; ). Identification of mycobacterial species by comparative sequence analysis of the RNA polymerase gene (rpoB). J Clin Microbiol 37, 1714–1720.
    [Google Scholar]
  7. Lévy-Frébault, V. V. & Portaels, F. ( 1992; ). Proposed minimal standards for the genus Mycobacterium and for description of slowly growing Mycobacterium species. Int J Syst Bacteriol 42, 315–323.[CrossRef]
    [Google Scholar]
  8. Portaels, F. ( 1995; ). Epidemiology of mycobacterial diseases. Clin Dermatol 13, 207–222.[CrossRef]
    [Google Scholar]
  9. Roth, A., Fischer, M., Hamid, M. E., Michalke, S., Ludwig, W. & Mauch, H. ( 1998; ). Differentiation of phylogenetically related slowly growing mycobacteria based on 16S–23S rRNA gene internal transcribed spacer sequences. J Clin Microbiol 36, 139–147.
    [Google Scholar]
  10. Roth, A., Reischl, U., Schonfeld, N., Naumann, L., Emler, S., Fischer, M., Mauch, H., Loddenkemper, R. & Kroppenstedt, R. M. ( 2000; ). Mycobacterium heckeshornense sp. nov., a new pathogenic slowly growing Mycobacterium sp. causing cavitary lung disease in an immunocompetent patient. J Clin Microbiol 38, 4102–4107.
    [Google Scholar]
  11. Springer, B., Stockman, L., Teschner, K., Roberts, G. D. & Bottger, E. C. ( 1996; ). Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods. J Clin Microbiol 34, 296–303.
    [Google Scholar]
  12. Telenti, A., Marchesi, F., Balz, M., Bally, F., Bottger, E. C. & Bodmer, T. ( 1993; ). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31, 175–178.
    [Google Scholar]
  13. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  14. Torkko, P., Suomalainen, S., Livanainen, E., Suutari, M., Tortoli, E., Paulin, L. & Katila, M.-L. ( 2000; ). Mycobacterium xenopi and related organisms isolated from stream waters in Finland and description of Mycobacterium botniense sp. nov. Int J Syst Evol Microbiol 50, 283–289.[CrossRef]
    [Google Scholar]
  15. Tortoli, E. ( 2003; ). Impact of genotypic studies on mycobacterial taxonomy: the new mycobacteria of the 1990s. Clin Microbiol Rev 16, 319–354.[CrossRef]
    [Google Scholar]
  16. Tortoli, E., Bartolini, A., Böttger, E. C., Emler, S., Garzelli, C., Magliano, E., Mantella, A., Rastogi, N., Rindi, L. & other authors ( 2001; ). Burden of unidentifiable mycobacteria in a reference laboratory. J Clin Microbiol 39, 4058–4065.[CrossRef]
    [Google Scholar]
  17. van Crevel, R., de Lange, W. C. M., Vanderpuye, N. A., van Soolingen, D., Hoogkamp-Korstanje, J. A. A., van Deuren, M., Kullberg, B. J., van Herwaarden, C. & van der Meer, J. W. ( 2001; ). The impact of nontuberculous mycobacteria on management of presumed pulmonary tuberculosis. Infection 29, 59–63.[CrossRef]
    [Google Scholar]
  18. van der Heijden, R. T., Snel, B., van Noort, V. & Huynen, M. A. ( 2007; ). Orthology prediction at scalable resolution by phylogenetic tree analysis. BMC Bioinformatics 8, 83 [CrossRef]
    [Google Scholar]
  19. van Ingen, J., Boeree, M. J., de Lange, W. C. M., Hoefsloot, W., Bendien, S. A., Magis-Escurra, C., Dekhuijzen, P. N. R. & van Soolingen, D. ( 2008; ). Mycobacterium xenopi clinical relevance and determinants, the Netherlands. Emerg Infect Dis 14, 385–389.[CrossRef]
    [Google Scholar]
  20. van Klingeren, B. & Pullen, W. ( 1993; ). Glutaraldehyde resistant mycobacteria from bronchoscope washers. J Hosp Infect 25, 147–149.[CrossRef]
    [Google Scholar]
  21. van Klingeren, B., Dessens-Kroon, M., van der Laan, T., Kremer, K. & van Soolingen, D. ( 2007; ). Drug susceptibility testing of Mycobacterium tuberculosis complex using a high throughput, reproducible, absolute concentration method. J Clin Microbiol 45, 2662–2668.[CrossRef]
    [Google Scholar]
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vol. , part 4, pp. 845 - 849

Phylogenetic relationship of the type strain of sp. nov. and related species of , based on gene sequences.

Phylogenetic relationship of the type strain of sp. nov. and related species of , based on gene sequences.

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