1887

Abstract

An aerobic, moderately halophilic, Gram-negative, motile, non-sporulating rod-shaped bacterium, designated strain A10, was isolated from the surface of leaves of the black mangrove and was subjected to a polyphasic taxonomic study. Strain A10 was able to grow at NaCl concentrations in the range 5–17.5 % (w/v) with optimum growth at 10 % (w/v) NaCl. Growth occurred at temperatures of 20–40 °C (optimal growth at 37 °C) and pH 5.5–8.5 (optimal growth at pH 7.0–8.0). The major respiratory quinone was ubiquinone 9. The major fatty acids were C, C 7, C cyclo 8 and C 3-OH. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, and unidentified phospholipids, glycolipids and an aminoglycolipid. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain A10 is closely related to MW2a (95.7 % sequence similarity), SW32 (95.2 %) and GC2.1 (95.0 %). Strain A10 formed a coherent phylogenetic branch with these three species, separated from other species of and closely related genera (with 16S rRNA gene sequence similarities below 94.0 %). A complete 23S rRNA gene sequence comparison of strain A10 with closely related species confirmed the phylogenetic position of the novel isolate, forming a branch with the species and , separated from other species of the genera belonging to the family (showing sequence similarities below 91.7 %). DNA–DNA hybridization studies between strain A10 and MW2a, DSM 15357 and CG2.1 were 21, 17 and 10 %, respectively. These levels of DNA–DNA relatedness were low enough to classify strain A10 as representing a genotypically distinct species. Overall, the phenotypic, genotypic, chemotaxonomic and phylogenetic results demonstrated that strain A10 represents a new genus and species. The name gen. nov., sp. nov. is proposed, with strain A10 (=CCM 7415=CECT 7220) as the type strain. This is the type species of the new proposed genus, which belongs to the family . In addition, our data support the placement of the species , and within this new genus, as comb. nov., comb. nov. and comb. nov., respectively.

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2009-02-01
2019-10-22
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