The taxonomic status of 24 haemolytic, non-glucose acidifying strains that did not belong to any previously described species was investigated by means of a polyphasic approach. Using AFLP fingerprinting, amplified rDNA restriction analysis and phenotypic characterization, the strains were classified into two phenetically coherent groups (comprising 15 and 9 strains) that were distinct from each other and from all known species. Confirmation that these groups formed two separate lineages within the genus was obtained from comparative analysis of partial sequences of the gene encoding the -subunit of RNA polymerase in all strains and also from 16S rRNA gene sequence analysis of representative strains. Previously published DNA–DNA reassociation data for some of the strains used also supported the species rank for both groups, for which the names sp. nov. and sp. nov. are proposed. The strains of sp. nov. originated from human and animal specimens and from various environmental sources, whereas those of sp. nov. were isolated exclusively from human clinical specimens. The phenotypic characteristics most useful for the differentiation of these species from other species that comprise haemolytic strains were the inability of sp. nov. to grow on -arginine and the ability of sp. nov. to grow on azelate. The type strain of sp. nov. is NIPH 838 (=LUH 4759=CCUG 51249=CCM 7266=58a) and the type strain of sp. nov. is NIPH 2150 (=RUH 422=CCUG 51248=CCM 7267=1271).


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Detailed data on the origins of the sp. nov. and sp. nov. strains. [PDF](21 KB)


Dendrogram derived from a cluster analysis of AFLP fingerprints. [PDF](22 KB)

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