1887

Abstract

Two Gram-negative strains of heterotrophic, aerobic, marine bacteria, designated PIM1 and PIN1, were isolated from seawater samples collected from the shallow coastal region of An-Ping Harbour, Tainan, Taiwan. Cells grown in broth cultures were straight rods and non-motile. The two isolates required NaCl for growth and grew optimally at 30–35 °C and 2–5 % NaCl. They grew aerobically and were not capable of anaerobic growth by fermentation of glucose or other carbohydrates. The cellular fatty acids were predominantly iso-branched, with iso-C (17.0–21.4 %), iso-C (18.2–21.0 %) and iso-C 9 (15.7–16.6 %) as the most abundant components. The predominant isoprenoid quinone was Q-8 (95.2–97.1 %). Strains PIM1 and PIN1 had DNA G+C contents of 46.6 and 46.9 mol%, respectively. Phylogeny based on 16S rRNA gene sequences and DNA–DNA hybridization, together with data from physiological, morphological and chemotaxonomic characterizations, indicated that the two isolates should be classified as representatives of two novel species of the genus of the family , for which the names sp. nov. (type strain PIM1=BCRC 17749=JCM 15083) and sp. nov. (type strain PIN1=BCRC 17750=JCM 15084) are proposed. In addition, based on the characterization data obtained in this study, it is proposed that and should be reclassified as comb. nov. and comb. nov., respectively.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.001180-0
2009-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/1/53.html?itemId=/content/journal/ijsem/10.1099/ijs.0.001180-0&mimeType=html&fmt=ahah

References

  1. Brettar, I., Christen, R. & Höfle, M. G.(2003).Idiomarina baltica sp. nov., a marine bacterium with a high optimum growth temperature isolated from surface water of the central Baltic Sea. Int J Syst Evol Microbiol 53, 407–413.[CrossRef] [Google Scholar]
  2. Choi, D. H. & Cho, B. C.(2005).Idiomarina seosinensis sp. nov., isolated from hypersaline water of a solar saltern in Korea. Int J Syst Evol Microbiol 55, 379–383.[CrossRef] [Google Scholar]
  3. Donachie, S. P., Hou, S., Gregory, T. S., Malahoff, A. & Alam, M.(2003).Idiomarina loihiensis sp. nov., a halophilic γ-Proteobacterium from the Lō‘ihi submarine volcano, Hawai‘i. Int J Syst Evol Microbiol 53, 1873–1879.[CrossRef] [Google Scholar]
  4. Fesefeldt, A., Kloos, K., Bothe, H., Lemmer, H. & Gliesche, C. G.(1998). Distribution of denitrification and nitrogen fixation genes in Hyphomicrobium spp. and other budding bacteria. Can J Microbiol 44, 181–186.[CrossRef] [Google Scholar]
  5. Hu, Z.-Y. & Li, Y.(2007).Pseudidiomarina sediminum sp. nov., a marine bacterium isolated from coastal sediments of Luoyuan Bay in China. Int J Syst Evol Microbiol 57, 2572–2577.[CrossRef] [Google Scholar]
  6. Ivanova, E. P., Romanenko, L. A., Chun, J., Matte, M. H., Matte, G. R., Mikhailov, V. V., Svetashev, V. I., Huq, A., Maugel, T. & Colwell, R. R.(2000).Idiomarina gen. nov., comprising novel indigenous deep-sea bacteria from the Pacific Ocean, including descriptions of two species, Idiomarina abyssalis sp. nov. and Idiomarina zobellii sp. nov. Int J Syst Evol Microbiol 50, 901–907.[CrossRef] [Google Scholar]
  7. Ivanova, E. P., Flavier, S. & Christen, R.(2004). Phylogenetic relationships among marine Alteromonas-like proteobacteria: emended description of the family Alteromonadaceae and proposal of Pseudoalteromonadaceae fam. nov., Colwelliaceae fam. nov., Shewanellaceae fam. nov., Moritellaceae fam. nov., Ferrimonadaceae fam. nov., Idiomarinaceae fam. nov. and Psychromonadaceae fam. nov. Int J Syst Evol Microbiol 54, 1773–1788.[CrossRef] [Google Scholar]
  8. Jean, W. D., Shieh, W. Y. & Chiu, H.-H.(2006).Pseudidiomarina taiwanensis gen. nov., sp. nov., a marine bacterium isolated from shallow coastal water of An-Ping Harbour, Taiwan, and emended description of the family Idiomarinaceae. Int J Syst Evol Microbiol 56, 899–905.[CrossRef] [Google Scholar]
  9. Kwon, S.-W., Kim, B.-Y., Weon, H.-Y., Baek, Y.-K., Koo, B.-S. & Go, S.-J.(2006).Idiomarina homiensis sp. nov., isolated from seashore sand in Korea. Int J Syst Evol Microbiol 56, 2229–2233.[CrossRef] [Google Scholar]
  10. Martínez-Cánovas, M. J., Béjar, V., Martínez-Checa, F., Páez, R. & Quesada, E.(2004).Idiomarina fontislapidosi sp. nov. and Idiomarina ramblicola sp. nov., isolated from inland hypersaline habitats in Spain. Int J Syst Evol Microbiol 54, 1793–1797.[CrossRef] [Google Scholar]
  11. Sasser, M.(1997).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  12. Shieh, W. Y. & Liu, C. M.(1996). Denitrification by a novel halophilic fermentative bacterium. Can J Microbiol 42, 507–514.[CrossRef] [Google Scholar]
  13. Shieh, W. Y., Chen, A.-L. & Chiu, H.-H.(2000).Vibrio aerogenes sp. nov., a facultatively anaerobic, marine bacterium that ferments glucose with gas production. Int J Syst Evol Microbiol 50, 321–329.[CrossRef] [Google Scholar]
  14. Shieh, W. Y., Lin, Y.-T. & Jean, W. D.(2004).Pseudovibrio denitrificans gen. nov., sp. nov., a marine, facultatively anaerobic, fermentative bacterium capable of denitrification. Int J Syst Evol Microbiol 54, 2307–2312.[CrossRef] [Google Scholar]
  15. Shieh, W. Y., Liu, T. Y., Lin, S. Y., Jean, W. D. & Chen, J.-S.(2008).Simiduia agarivorans gen. nov., sp. nov., a marine agarolytic bacterium isolated from shallow coastal water from Keelung, Taiwan. Int J Syst Evol Microbiol 58, 895–900.[CrossRef] [Google Scholar]
  16. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  17. Yoon, J.-H., Jung, S.-Y., Jung, Y.-T. & Oh, T.-K.(2007).Idiomarina salinarum sp. nov., isolated from a marine solar saltern in Korea. Int J Syst Evol Microbiol 57, 2503–2506.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.001180-0
Loading
/content/journal/ijsem/10.1099/ijs.0.001180-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error