@article{mbs:/content/journal/ijsem/10.1099/ijs.0.001123-0, author = "Vanlaere, Elke and Baldwin, Adam and Gevers, Dirk and Henry, Deborah and De Brandt, Evie and LiPuma, John J. and Mahenthiralingam, Eshwar and Speert, David P. and Dowson, Chris and Vandamme, Peter", title = "Taxon K, a complex within the Burkholderia cepacia complex, comprises at least two novel species, Burkholderia contaminans sp. nov. and Burkholderia lata sp. nov.", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2009", volume = "59", number = "1", pages = "102-111", doi = "https://doi.org/10.1099/ijs.0.001123-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.001123-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "MLST, multilocus sequence typing", keywords = "RFLP, restriction fragment length polymorphism", keywords = "ANI, average nucleotide identity", keywords = "ST, sequence type", keywords = "Bcc, Burkholderia cepacia complex", abstract = "The aim of the present study was to re-examine the taxonomic position and structure of taxon K (also known as group K) within the Burkholderia cepacia complex (Bcc). For this purpose, a representative set of strains was examined by a traditional polyphasic taxonomic approach, by multilocus sequence typing (MLST) analysis and by analysis of available whole-genome sequences. Analysis of the recA gene sequence revealed three different lineages, designated recA-I, recA-II and recA-III. DNA–DNA hybridization experiments demonstrated that recA-I and recA-II isolates each represented a single novel species. However, DNA–DNA hybridization values of recA-II strains towards recA-III strains and among recA-III strains were at the threshold level for species delineation. By MLST, recA-I isolates were clearly distinguished from the others and represented a distinct lineage referred to as MLST-I, whereas recA-II and recA-III isolates formed a second MLST lineage referred to as MLST-II. A divergence value of 3.5 % was obtained when MLST-I was compared with MLST-II. The internal level of concatenated sequence divergence within MLST-I and MLST-II was 1.4 and 2.7 %, respectively; by comparison with the level of concatenated sequence divergence in established Bcc species, these data demonstrate that the MLST-I and MLST-II lineages represent two distinct species within the Bcc. The latter conclusion was supported by comparison of the whole-genome average nucleotide identity (ANI) level of MLST-I and MLST-II strains with strains of established Bcc species and by a whole-genome-based phylogenetic analysis. We formally propose to classify taxon K bacteria from the MLST-I and MLST-II lineages as Burkholderia contaminans sp. nov. (with strain J2956T =LMG 23361T =CCUG 55526T as the type strain) and Burkholderia lata sp. nov. (with strain 383T =ATCC 17760T =LMG 22485T =CCUG 55525T as the type strain), respectively. The MLST approach was confirmed as a valuable instrument in polyphasic taxonomic studies; more importantly, the cumulative data for about 1000 Bcc isolates analysed demonstrate that the 3 % concatenated sequence divergence level correlates with the 70 % DNA–DNA hybridization or 95 % whole-genome ANI threshold levels for species delineation.", }