1887

Abstract

It has been recognized that there is heterogeneity among isolates in culture collections worldwide, causing confounding results for researchers utilizing these organisms; one such culture is serovar Perameles. The serovar reference strain Bandicoot 343 was previously identified to the species level by DNA–DNA hybridization; however, subsequent published studies demonstrated results that contradicted the initial speciation. In this study, initial serological testing was performed with isolates from the culture collections of the Centers for Disease Control (CDC), Atlanta, USA (strain Lepto0214), and the WHO/FAO/OIE Collaborating Centre for Reference and Research on Leptospirosis, Brisbane, Australia (strain Bandicoot 343), and the original serovar Perameles hyperimmune antiserum produced in 1964. The results indicated that strain Lepto0214 was not serologically reactive to the antiserum. However, further investigations revealed an alternative serovar Perameles strain held in the CDC collection (Lepto0213) that yielded titres against the antiserum. 16S rRNA gene sequencing of the three strains revealed that Lepto0214 had significant sequence similarity with previously sequenced strains; however, strains Lepto0213 and Bandicoot 343 had significant similarity with strains. 16S rRNA gene sequencing results were confirmed by pulsed-field gel electrophoresis; Lepto0214 had a pattern similar to that of serovar Hardjo strain Went 5, and the pattern differed significantly from those of Lepto0213 and Bandicoot 343. This research provides evidence for the reclassification of serovar Perameles from to . This reclassification highlights a need for changes to how reference serovars are identified, disseminated and stored, with the aim of reducing heterogeneity of reference strains between culture collections.

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2009-05-01
2019-12-11
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DNA sequence alignment of the 16S rRNA gene sequences of strains Lepto0213, Lepto0214 and Bandicoot 343 and strains of 20 representative species and genomospecies. The alignment was constructed using the CLUSTAL W algorithm. [MEGA format][Plain text format](33 KB)

DNA sequence alignment of the 16S rRNA gene sequences of strains Lepto0213, Lepto0214 and Bandicoot 343 and strains of 20 representative species and genomospecies. The alignment was constructed using the CLUSTAL W algorithm. [MEGA format][Plain text format](33 KB)

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16S rRNA gene sequence-based phylogeny of strains Lepto0213, Lepto0214 and Bandicoot 343 and strains of 20 representative species and genomospecies. [PDF](18 KB)

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