1887

Abstract

A novel, Gram-negative bacterium, designated CS5-B2, was isolated from soil that had been collected from a cliff on Mara Island, Republic of Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the radiation of the genus . The closest relatives were the type strains of , and and 16S rRNA gene sequence similarities between strain CS5-B2 and these strains were 99.0, 97.9 and 97.8 %, respectively. The sequence similarities between the novel isolate and other related taxa compared in the phylogenetic analysis were less than 96.7 %. The cells of strain CS5-B2 were aerobic, oxidase-negative, catalase-positive, motile rods. The temperature range for growth was 20–37 °C, with optimal growth at 30–37 °C. Growth occurred at pH 5.1–9.1, with optimal growth at pH 6.1–9.1. NaCl tolerance for growth was from 1  to 2 % (w/v). Ubiquinone-8 was the predominant respiratory lipoquinone. The major fatty acids were iso-C and iso-C 9. The G+C content of the DNA was 65.7–66.6 mol%. The level of DNA–DNA relatedness with KCTC 12359 was 20.2 and 29.6 % in duplicate measurements. On the basis of phenotypic features, phylogenetic analysis and DNA–DNA relatedness, a novel species of the genus is proposed, with the name sp. nov. The type strain is CS5-B2 (=JCM 14959 =KCTC 22144).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000968-0
2009-06-01
2019-10-17
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/6/1397.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000968-0&mimeType=html&fmt=ahah

References

  1. An, D.-S., Im, W.-T., Yang, H.-C., Yang, D.-C. & Lee, S.-T. ( 2005; ). Dyella koreensis sp. nov., a β-glucosidase-producing bacterium. Int J Syst Evol Microbiol 55, 1625–1628.[CrossRef]
    [Google Scholar]
  2. Collins, M. D. ( 1985; ). Analysis of isoprenoid quinones. Methods Microbiol 18, 329–366.
    [Google Scholar]
  3. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  5. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M. & Schrempf, H. ( 1985; ). Genetic Manipulation of Streptomyces: a Laboratory Manual. Norwich: John Innes Foundation.
  7. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  8. Jung, H.-M., Ten, L. N., Kim, K.-H., An, D. S., Im, W.-T. & Lee, S.-T. ( 2009; ). Dyella ginsengisoli sp. nov., isolated from soil of a ginseng field in South Korea. Int J Syst Evol Microbiol 59, 460–465.[CrossRef]
    [Google Scholar]
  9. Kim, B.-Y., Weon, H.-Y., Lee, K.-H., Seok, S.-J., Kwon, S.-W., Go, S.-J. & Stackebrandt, E. ( 2006; ). Dyella yeojuensis sp. nov., isolated from greenhouse soil in Korea. Int J Syst Evol Microbiol 56, 2079–2082.[CrossRef]
    [Google Scholar]
  10. Kroppenstedt, R. M. ( 1985; ). Fatty acid and menaquinone analysis of actinomycetes and related organisms. In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by M. Goodfellow & D. E. Minnikin. New York: Academic Press.
  11. Lee, D. W. & Lee, S. D. ( 2008; ). Aeromicrobium ponti sp. nov., isolated from seawater. Int J Syst Evol Microbiol 58, 987–991.[CrossRef]
    [Google Scholar]
  12. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  13. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  14. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  15. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  16. Xie, C.-H. & Yokota, A. ( 2005; ). Dyella japonica gen. nov., sp. nov., a gamma-proteobacterium isolated from soil. Int J Syst Evol Microbiol 55, 753–756.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000968-0
Loading
/content/journal/ijsem/10.1099/ijs.0.000968-0
Loading

Data & Media loading...

Supplements

Cell morphology of strain CS5-B2 as determined with a transmission electron microscope. [ PDF] 249 KB

PDF

Cellular fatty acid contents of strain CS5-B2 and the type strains of and [ PDF] 77 KB

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error