A novel, Gram-negative bacterium, designated CS5-B2, was isolated from soil that had been collected from a cliff on Mara Island, Republic of Korea. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain fell within the radiation of the genus . The closest relatives were the type strains of , and and 16S rRNA gene sequence similarities between strain CS5-B2 and these strains were 99.0, 97.9 and 97.8 %, respectively. The sequence similarities between the novel isolate and other related taxa compared in the phylogenetic analysis were less than 96.7 %. The cells of strain CS5-B2 were aerobic, oxidase-negative, catalase-positive, motile rods. The temperature range for growth was 20–37 °C, with optimal growth at 30–37 °C. Growth occurred at pH 5.1–9.1, with optimal growth at pH 6.1–9.1. NaCl tolerance for growth was from 1  to 2 % (w/v). Ubiquinone-8 was the predominant respiratory lipoquinone. The major fatty acids were iso-C and iso-C 9. The G+C content of the DNA was 65.7–66.6 mol%. The level of DNA–DNA relatedness with KCTC 12359 was 20.2 and 29.6 % in duplicate measurements. On the basis of phenotypic features, phylogenetic analysis and DNA–DNA relatedness, a novel species of the genus is proposed, with the name sp. nov. The type strain is CS5-B2 (=JCM 14959 =KCTC 22144).


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Cell morphology of strain CS5-B2 as determined with a transmission electron microscope. [ PDF] 249 KB


Cellular fatty acid contents of strain CS5-B2 and the type strains of and [ PDF] 77 KB


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