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Volume 59, Issue 3

Emended description of and descriptions of sp. nov. and sp. nov., previously identified as genospecies 1, 2 and WVA 963 Open Access

Abstract

is an important early colonizer in the oral biofilm and consists of three genospecies (1, 2 and WVA 963) which cannot be readily differentiated using conventional phenotypic testing or on the basis of 16S rRNA gene sequencing. We have investigated a representative collection of type and reference strains and clinical and oral isolates (=115) and determined the partial gene sequences of six housekeeping genes (, , , , and ). These sequences identified the three genospecies and differentiated them from isolated from rodents. The partial sequences of and gave best separation of the three genospecies. genospecies 1 and 2 formed two distinct clusters, well separated from both genospecies WVA 963 and . Analysis of the same genes in other oral species (, , , and ) indicated that, when sequence data were obtained, these species each exhibited <90 % similarity with the genospecies. Based on these data, we propose the name sp. nov. (type strain ATCC 27044 =CCUG 34288) for genospecies 2 and sp. nov. (type strain ATCC 49338 =CCUG 34287) for genospecies WVA 963. genospecies 1 should remain as , with the type strain ATCC 12104 =NCTC 10301 =CCUG 2238.

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2009-03-01
2024-04-16
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Emended description of Actinomyces naeslundii and descriptions of Actinomyces oris sp. nov. and Actinomyces johnsonii sp. nov., previously identified as Actinomyces naeslundii genospecies 1, 2 and WVA 963
Int J Syst Evol Microbiol 59, 509 (2009); https://doi.org/10.1099/ijs.0.000950-0
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vol. , part 3, pp. 509 - 516

16S rRNA gene sequence similarity between groups

Strains used and accession numbers for partial 16S rRNA gene sequences

Details of oral and non-oral isolates and reference and type strains used in this study and GenBank accession numbers of partial sequences

Phylogenetic relationships between genospecies 1 ( ), genospecies 2 ( sp. nov.) and serotype WVA 963 ( sp. nov.), generated by neighbour-joining analysis of partial gene sequences of , , and

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