1887

Abstract

A Gram-negative, ovoid rod-shaped bacterium that lacked bacteriochlorophyll , designated strain Y-2, was isolated from seawater of the Taiwan Strait, China. The strain was oxidase- and catalase-positive, and reduced nitrate to nitrite. Optimal growth occurred at around pH 7.0 and 25 °C in the presence of 1–3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Y-2 belonged to the class and formed a coherent cluster with SW-277 (96.2 % sequence similarity). The major whole-cell fatty acid was C 7 (75.42 %). Other characteristic fatty acids were C (9.16 %) and C (7.32 %). The DNA G+C content of strain Y-2 was 62.4 mol%. Therefore, based on phenotypic properties, phylogeny and genomic data, strain Y-2 should be placed in the genus as representing a novel species, for which the name sp. nov. is proposed. The type strain is Y-2 (=MCCC 1A00107=LMG 24574=CGMCC 1.7081).

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2009-05-01
2020-01-24
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References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors) ( 1995; ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley.
  2. Bruns, A., Rohde, M. & Berthe-Corti, L. ( 2001; ). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. Int J Syst Evol Microbiol 51, 1997–2006.[CrossRef]
    [Google Scholar]
  3. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  4. Ivanova, E. P., Kiprianova, E. A., Mikhailov, V. V., Levanova, G. F., Garagulya, A. D., Gorshkova, N. M., Vysotskii, M. V., Nicolau, D. V., Yumoto, N. & other authors ( 1998; ). Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48, 247–256.[CrossRef]
    [Google Scholar]
  5. Keswani, J. & Whitman, W. B. ( 2001; ). Relationship of 16S rRNA sequence similarity to DNA hybridization in prokaryotes. Int J Syst Evol Microbiol 51, 667–678.
    [Google Scholar]
  6. Lafay, B., Ruimy, R., Rausch de Traubenberg, C., Breittmayer, V., Gauthier, M. J. & Christen, R. ( 1995; ). Roseobacter algicola sp. nov., a new marine bacterium isolated from the phycosphere of the toxin-producing dinoflagellate Prorocentrum lima. Int J Syst Bacteriol 45, 290–296.[CrossRef]
    [Google Scholar]
  7. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  8. Liu, C. & Shao, Z. ( 2005; ). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55, 1181–1186.[CrossRef]
    [Google Scholar]
  9. Martínez-Cánovas, M. J., Quesada, E., Martínez-Checa, F., del Moral, A. & Béjar, V. ( 2004; ). Salipiger mucescens gen. nov., sp. nov., a moderately halophilic, exopolysaccharide-producing bacterium isolated from hypersaline soil, belonging to the a-Proteobacteria. Int J Syst Evol Microbiol 54, 1735–1740.[CrossRef]
    [Google Scholar]
  10. Mesbah, M. & Whitman, W. B. ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef]
    [Google Scholar]
  11. Mrozik, A., Piotrowska-Seget, Z. P. & Labuzek, S. ( 2004; ). Changes in whole cell-derived fatty acids induced by naphthalene in bacteria from genus Pseudomonas. Microbiol Res 159, 87–95.[CrossRef]
    [Google Scholar]
  12. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  13. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  14. Shieh, W. Y., Chen, Y.-W., Chaw, S.-M. & Chiu, H.-H. ( 2003; ). Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53, 479–484.[CrossRef]
    [Google Scholar]
  15. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  16. Suzuki, T., Muroga, Y., Takahama, M. & Nishimura, Y. ( 1999; ). Roseivivax halodurans gen. nov., sp. nov. and Roseivivax halotolerans sp. nov., aerobic bacteriochlorophyll-containing bacteria isolated from a saline lake. Int J Syst Bacteriol 49, 629–634.[CrossRef]
    [Google Scholar]
  17. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  18. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  19. Yoon, J.-H., Kang, S.-J. & Oh, T.-K. ( 2007; ). Donghicola eburneus gen. nov., sp. nov., isolated from seawater of the East Sea in Korea. Int J Syst Evol Microbiol 57, 73–76.[CrossRef]
    [Google Scholar]
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Transmission electron micrographs of negatively stained cells of strain Y . [PDF](68 KB)

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Cellular fatty acid contents of strain Y-2 , SW-277 and A3 . [PDF](16 KB)

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