A novel, non-pigmented, slow-growing mycobacterium was identified on the basis of biochemical and nucleic acid analyses, as well as growth characteristics. Three isolates were cultured from clinical samples (two from sputum and one from pus in lymph nodes) obtained from three immunocompetent patients with infections. Bacterial growth occurred at 28–42 °C on Middlebrook 7H11-OADC agar. The isolates showed negative results for Tween hydrolysis, nitrate reductase, semiquantitative catalase, urease activity, 3 day arylsulfatase activity, pyrazinamidase, tellurite reduction and niacin accumulation tests, but positive results for 14 day arylsulfatase activity and heat-stable catalase tests. The isolates contained -, keto-, and dicarboxymycolates in their cell walls. Sequence analysis revealed that all isolates had identical, unique 16S rRNA sequences. Phylogenetic analysis of the 16S rRNA, , and gene sequences confirmed that these isolates are unique but closely related to . DNA–DNA hybridization of the isolates demonstrated less than 50 % reassociation with and . On the basis of these findings, a novel species designated sp. nov. is proposed. The type strain is KUM 060204 (=JCM 15038=DSM 45166).


Article metrics loading...

Loading full text...

Full text loading...



  1. Boor, K. J., Duncan, M. L. & Price, C. W.(1995). Genetic and transcriptional organization of the region encoding the beta subunit of Bacillus subtilis RNA polymerase. J Biol Chem 270, 20329–20336.[CrossRef] [Google Scholar]
  2. Brander, E., Jantzen, E., Huttunen, R., Julkunen, A. & Katila, M. L.(1992). Characterization of a distinct group of slowly growing mycobacteria by biochemical tests and lipid analyses. J Clin Microbiol 30, 1972–1975. [Google Scholar]
  3. Brown-Elliott, B. & Wallace, R. J., Jr(2005). Infections caused by nontuberculous mycobacteria. In Principles and Practice of Infectious Diseases, vol. 2, pp. 2909–2915. Edited by G. L. Mandell, J. E. Bennett & R. Dolin. Pennsylvania: Elsevier.
  4. Butler, W. R., O'Connor, S. P., Yakrus, M. A., Smithwick, R. W., Plikaytis, B. B., Moss, C. W., Floyd, M. M., Woodley, C. L., Kilburn, J. O. & other authors(1993).Mycobacterium celatum sp. nov. Int J Syst Bacteriol 43, 539–548.[CrossRef] [Google Scholar]
  5. Chapin, K. C.(2007). Principles of stains and media. In Manual of Clinical Microbiology, 9th edn, pp. 182–191. Edited by P. R. Murray, E. J. Baron, M. L. Landry, J. H. Jorgensen & M. A. Pfaller. Washington, DC: American Society for Microbiology.
  6. Domenech, P., Jimenez, M. S., Menendez, M. C., Bull, T. J., Samper, S., Manrique, A. & Garcia, M. J.(1997).Mycobacterium mageritense sp. nov. Int J Syst Bacteriol 47, 535–540.[CrossRef] [Google Scholar]
  7. Ezaki, T., Hashimoto, Y., Takeuchi, N., Yamamoto, H., Liu, S. L., Miura, H., Matsui, K. & Yabuuchi, E.(1988). Simple genetic method to identify viridans group streptococci by colorimetric dot hybridization and fluorometric hybridization in microdilution wells. J Clin Microbiol 26, 1708–1713. [Google Scholar]
  8. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid–deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Evol Microbiol 39, 224–229. [Google Scholar]
  9. Kent, P. T. & Kubica, G. P.(1985).Public Health Mycobacteriology: a Guide for the Level III Laboratory. Atlanta: US Department of Health and Human Services, Centers for Disease Control.
  10. Kirschner, P., Springer, B., Vogel, U., Meier, A., Wrede, A., Kiekenbeck, M., Bange, F. C. & Bottger, E. C.(1993). Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clinical laboratory. J Clin Microbiol 31, 2882–2889. [Google Scholar]
  11. Koukila-Kahkola, P., Springer, B., Bottger, E. C., Paulin, L., Jantzen, E. & Katila, M. L.(1995).Mycobacterium branderi sp. nov., a new potential human pathogen. Int J Syst Bacteriol 45, 549–553.[CrossRef] [Google Scholar]
  12. Li, Y., Kawamura, Y., Fujiwara, N., Naka, T., Liu, H., Huang, X., Kobayashi, K. & Ezaki, T.(2004).Sphingomonas yabuuchiae sp. nov. and Brevundimonas nasdae sp. nov., isolated from the Russian space laboratory Mir. Int J Syst Evol Microbiol 54, 819–825.[CrossRef] [Google Scholar]
  13. Masaki, T., Ohkusu, K., Hata, H., Fujiwara, N., Iihara, H., Yamada-Noda, M., Nhung, P. P., Hayashi, M., Asano, Y. & other authors(2006).Mycobacterium kumamotonense sp. nov. recovered from clinical specimen and the first isolation report of Mycobacterium arupense in Japan: novel slowly growing, nonchromogenic clinical isolates related to Mycobacterium terrae complex. Microbiol Immunol 50, 889–897.[CrossRef] [Google Scholar]
  14. Medical Section of the American Lung Association(1997). Diagnosis and treatment of disease caused by nontuberculous mycobacteria. This official statement of the American Thoracic Society was approved by the Board of Directors, March 1997. Am J Respir Crit Care Med 156, S1–S25.[CrossRef] [Google Scholar]
  15. NCCLS(2003).Susceptibility Testing of Mycobacteria, Nocardiae, and Other Aerobic Actinomycetes; Approved Standard. NCCLS document M24-A. Wayne, PA: National Committee for Clinical Laboratory Standards.
  16. Telenti, A., Marchesi, F., Balz, M., Bally, F., Bottger, E. C. & Bodmer, T.(1993). Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol 31, 175–178. [Google Scholar]
  17. Vincent, V. & Gutierres, M. C.(2007). Mycobacterium: laboratory characteristics of slowly growing mycobacteria. In Manual of Clinical Microbiology, 9th edn, pp. 573–588. Edited by P. R. Murray, E. J. Baron, M. L. Landry, J. H. Jorgensen & M. A. Pfaller. Washington, DC: American Society for Microbiology.

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error