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Abstract

Two strains of psychrotolerant bacteria, designated M7 and M5, isolated from Antarctic coastal marine environments were studied to determine their taxonomic position. The organisms comprised Gram-negative, rod-shaped, facultatively anaerobic cells that were motile by means of single polar flagella. Neither of the bacterial isolates had a requirement for Na. These two psychrotolerant strains grew at temperatures ranging from −4 to 30 °C. Both strains were capable of producing HS from thiosulfate and were able to use sodium nitrate and trimethylamine -oxide as terminal electron acceptors during anaerobic growth. 16S rRNA gene sequence analysis placed M7 and M5 within the genus ; the strains showed the highest similarity (99.9 and 99.2 % respectively) with respect to the type strains of and . However the levels of sequence similarity between strain M7 and the type strains of . and were 87.6 and 87.4 %, respectively. DNA–DNA hybridization experiments performed between the Antarctic isolate M7 and LMG 19866 and LMG 19475 revealed levels of relatedness of 32 and 35 %, respectively. Strain M5 showed 100 % DNA relatedness with respect to strain M7. The DNA G+C content of these bacteria was 42 mol%. Several phenotypic characteristics, the cellular fatty acid compositions and the quinone content of strains M7 and M5 served to differentiate them from related shewanellae. On the basis of the data from this polyphasic taxonomic study, M7 and M5 constitute a single genospecies. They represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is M7 (=LMG 24424 =CECT 7339).

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2009-02-01
2020-01-17
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Transmission electron micrographs of ultrathin sections from sp. nov. M7 prepared by means of high-pressure freezing and freeze-substitution. (left) General view of the cells, showing large numbers of membrane vesicles around them. (right) Magnified view, in which the two-layered structure of a membrane vesicle protruding from the cell is clearly visible. Bars, 500 nm (left) and 100 nm (right).

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Transmission electron micrographs of ultrathin sections from sp. nov. M7 prepared by means of high-pressure freezing and freeze-substitution. (left) General view of the cells, showing large numbers of membrane vesicles around them. (right) Magnified view, in which the two-layered structure of a membrane vesicle protruding from the cell is clearly visible. Bars, 500 nm (left) and 100 nm (right).

IMAGE

Cellular fatty acid compositions for strains M7 and M5. [PDF](20 KB)

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