A novel actinomycete strain, designated CW 108, was isolated from a forest soil in Anhui Province, China. The cells were strictly aerobic, non-motile, bent rods. The strain grew optimally at 30–37 °C and pH 6.0–8.0. Chemotaxonomically, the peptidoglycan type was A3, the cell-wall sugars contained galactose, mannose and ribose, the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylmonomethylethanolamine, the major fatty acids were ai-C, i-C and ai-C and the predominant respiratory quinone was MK-9(H), with a moderate amount of MK-8(H) and a minor amount of MK-6(H). The G+C content of the genomic DNA was 71.1 mol%. Phylogenetic analysis showed that strain CW 108 formed a deeply separated lineage in the family with DSM 20127. The 16S rRNA gene sequence similarity between strain CW 108 and DSM 20127 was 99.4 %; sequence similarities between these strains and representatives of other species in the family with validly published names were lower than 95.2 %. DNA–DNA hybridization showed a level of relatedness of 52.2 % between the two neighbours. Based on the results of our polyphasic taxonomic study, strain CW 108 and DSM 20127 should be assigned to two different species of a novel genus within the family , for which the names gen. nov., sp. nov. and comb. nov. are proposed. The type strain of is CW 108 (=LMG 24447 =KCTC 19388 =CCTCC AB 207194) and the type strain of is DSM 20127 =JCM 1329 =CGMCC 1.1891 =ATCC 13752.


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Transmission electron micrographs TEM of cells of strain CW 108 grown on PYES agar (pH 7.0±0.5) for 2 days at 30 °C. Bars, 0.5 µm (a); 1.0 µm (b).


Transmission electron micrographs TEM of cells of strain CW 108 grown on PYES agar (pH 7.0±0.5) for 2 days at 30 °C. Bars, 0.5 µm (a); 1.0 µm (b).


Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences, showing the positions of strains CW 108 and DSM 20127 . [PDF](18 KB)


Two-dimensional TLC of a polar-lipid extract from strain CW 108 stained with molybdenum blue. DPG, Diphosphatidylglycerol; PG, diphosphatidylglycerol; PI, phosphatidylinositol; PME, phosphatidylmonomethylethanolamine.


Colony morphologies for strains CW 108 and DSM 20127 grown on YDC agar (pH 7.0±0.5) for 2 days at 30 °C.

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