1887

Abstract

A novel actinomycete strain, designated CW 108, was isolated from a forest soil in Anhui Province, China. The cells were strictly aerobic, non-motile, bent rods. The strain grew optimally at 30–37 °C and pH 6.0–8.0. Chemotaxonomically, the peptidoglycan type was A3, the cell-wall sugars contained galactose, mannose and ribose, the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylmonomethylethanolamine, the major fatty acids were ai-C, i-C and ai-C and the predominant respiratory quinone was MK-9(H), with a moderate amount of MK-8(H) and a minor amount of MK-6(H). The G+C content of the genomic DNA was 71.1 mol%. Phylogenetic analysis showed that strain CW 108 formed a deeply separated lineage in the family with DSM 20127. The 16S rRNA gene sequence similarity between strain CW 108 and DSM 20127 was 99.4 %; sequence similarities between these strains and representatives of other species in the family with validly published names were lower than 95.2 %. DNA–DNA hybridization showed a level of relatedness of 52.2 % between the two neighbours. Based on the results of our polyphasic taxonomic study, strain CW 108 and DSM 20127 should be assigned to two different species of a novel genus within the family , for which the names gen. nov., sp. nov. and comb. nov. are proposed. The type strain of is CW 108 (=LMG 24447 =KCTC 19388 =CCTCC AB 207194) and the type strain of is DSM 20127 =JCM 1329 =CGMCC 1.1891 =ATCC 13752.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000695-0
2009-02-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/2/259.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000695-0&mimeType=html&fmt=ahah

References

  1. Altenburger, P., Kämpfer, P., Schumann, P., Steiner, R., Lubitz, W. & Busse, H.-J. ( 2002; ). Citricoccus muralis gen. nov., sp. nov., a novel actinobacterium isolated from a medieval wall painting. Int J Syst Evol Microbiol 52, 2095–2100.[CrossRef]
    [Google Scholar]
  2. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  3. Felsenstein, J. ( 1985; ). Conference limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Hu, H. Y., Lim, B. R., Naohiro, G. & Koich, F. J. ( 2001; ). Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. J Microbiol Methods 47, 17–24.[CrossRef]
    [Google Scholar]
  5. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  6. Interschick, E., Fiedler, F., Schleifer, K. H. & Kandler, O. ( 1970; ). Glycine amide: a constituent of the murein of Arthrobacter atrocyaneus. Z Naturforsch B 25, 714–717.
    [Google Scholar]
  7. Jahnke, K. D. ( 1992; ). basic computer program for evaluation of spectroscopic DNA renaturation data from Gilford System 2600 spectrophotometer on a PC/XT/AT type personal computer. J Microbiol Methods 15, 61–73.[CrossRef]
    [Google Scholar]
  8. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  10. Koch, C., Schumann, P. & Stackebrandt, E. ( 1995; ). Reclassification of Micrococcus agilis (Ali-Cohen 1889) to the genus Arthrobacter as Arthrobacter agilis comb. nov. and emendation of the genus Arthrobacter. Int J Syst Bacteriol 45, 837–839.[CrossRef]
    [Google Scholar]
  11. Kuhn, D. A. & Starr, M. P. ( 1960; ). Arthrobacter atrocyaneus n. sp. and its blue pigment. Arch Mikrobiol 36, 175–181.[CrossRef]
    [Google Scholar]
  12. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  13. Li, W. J., Chen, H. H., Zhang, Y. Q., Kim, C. J., Park, D. J., Lee, J. C., Xu, L. H. & Jiang, C. L. ( 2005; ). Citricoccus alkalitolerans sp. nov., a novel actinobacterium isolated from a desert soil in Egypt. Int J Syst Evol Microbiol 55, 87–90.[CrossRef]
    [Google Scholar]
  14. Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L. ( 2007; ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef]
    [Google Scholar]
  15. Liu, H., Xu, Y., Ma, Y. & Zhou, P. ( 2000; ). Characterization of Micrococcus antarcticus sp. nov., a psychrophilic bacterium from Antarctica. Int J Syst Evol Microbiol 50, 715–719.[CrossRef]
    [Google Scholar]
  16. Liu, X. Y., Wang, B. J., Jiang, C. Y. & Liu, S. J. ( 2007; ). Micrococcus flavus sp. nov., isolated from activated sludge in a bioreactor. Int J Syst Evol Microbiol 57, 66–69.[CrossRef]
    [Google Scholar]
  17. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  18. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  19. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H. ( 1984; ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef]
    [Google Scholar]
  20. Pukall, R., Schumann, P., Schütte, C., Gols, R. & Dicke, M. ( 2006; ). Acaricomes phytoseiuli gen. nov., sp. nov., isolated from the predatory mite Phytoseiulus persimilis. Int J Syst Evol Microbiol 56, 465–469.[CrossRef]
    [Google Scholar]
  21. Sanders, J. E. & Fryer, J. L. ( 1980; ). Renibacterium salmoninarum gen. nov., sp. nov., the causative agent of bacterial kidney disease in salmonid fishes. Int J Syst Bacteriol 30, 496–502.[CrossRef]
    [Google Scholar]
  22. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16
    [Google Scholar]
  23. Schleifer, K. H. & Kandler, O. ( 1972; ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477.
    [Google Scholar]
  24. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  25. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  26. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  27. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  28. Wieser, M., Denner, E. B. M., Kämpfer, P., Schumann, P., Tindall, B., Steiner, U., Vybiral, D., Lubitz, W., Maszenan, A. M. & other authors ( 2002; ). Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). Int J Syst Evol Microbiol 52, 629–637.
    [Google Scholar]
  29. Yamada, K. & Komagata, K. ( 1972; ). Taxonomic studies on coryneform bacteria. V. Classification of coryneform bacteria. J Gen Appl Microbiol 18, 417–431.[CrossRef]
    [Google Scholar]
  30. Yoon, J. H., Kim, I. G., Kang, K. H., Oh, T. K. & Park, Y. H. ( 2003; ). Bacillus marisflavi sp. nov. and Bacillus aquimaris sp. nov., isolated from sea water of a tidal flat of the Yellow Sea in Korea. Int J Syst Evol Microbiol 53, 1297–1303.[CrossRef]
    [Google Scholar]
  31. Zhang, Y. Q., Schumann, P., Yu, L. Y., Liu, H. Y., Zhang, Y. Q., Xu, L. H., Stackebrandt, E., Jiang, C. L. & Li, W. J. ( 2007; ). Zhihengliuella halotolerans gen. nov., sp. nov., a novel member of the family Micrococcaceae. Int J Syst Evol Microbiol 57, 1018–1023.[CrossRef]
    [Google Scholar]
  32. Zhou, Y., Dong, J., Wang, X., Huang, X., Zhang, K. Y., Zhang, Y. Q., Guo, Y. F., Lai, R. & Li, W. J. ( 2007; ). Chryseobacterium flavum sp. nov., isolated from a polluted soil. Int J Syst Evol Microbiol 57, 1765–1769.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000695-0
Loading
/content/journal/ijsem/10.1099/ijs.0.000695-0
Loading

Data & Media loading...

Supplements

Transmission electron micrographs TEM of cells of strain CW 108 grown on PYES agar (pH 7.0±0.5) for 2 days at 30 °C. Bars, 0.5 µm (a); 1.0 µm (b).

IMAGE

Transmission electron micrographs TEM of cells of strain CW 108 grown on PYES agar (pH 7.0±0.5) for 2 days at 30 °C. Bars, 0.5 µm (a); 1.0 µm (b).

IMAGE

Maximum-parsimony phylogenetic tree based on 16S rRNA gene sequences, showing the positions of strains CW 108 and DSM 20127 . [PDF](18 KB)

PDF

Two-dimensional TLC of a polar-lipid extract from strain CW 108 stained with molybdenum blue. DPG, Diphosphatidylglycerol; PG, diphosphatidylglycerol; PI, phosphatidylinositol; PME, phosphatidylmonomethylethanolamine.

IMAGE

Colony morphologies for strains CW 108 and DSM 20127 grown on YDC agar (pH 7.0±0.5) for 2 days at 30 °C.

IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error