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Volume 59, Issue 2

sp. nov., a thermophilic, hydrogenogenic, carboxydotrophic, dissimilatory Fe(III)-reducing bacterium from a Kamchatka hot spring Free

Abstract

A novel anaerobic, thermophilic, Fe(III)-reducing, CO-utilizing bacterium, strain 1315, was isolated from a hot spring of Geyser Valley on the Kamchatka Peninsula. Cells of the new isolate were Gram-positive, short rods. Growth was observed at 52–70 °C, with an optimum at 65 °C, and at pH 5.5–8.5, with an optimum at pH 6.5–7.2. In the presence of Fe(III) or 9,10-anthraquinone 2,6-disulfonate (AQDS), the bacterium was capable of growth with CO and yeast extract (0.2 g l); during growth under these conditions, strain 1315 produced H and CO and Fe(II) or AQDSH, respectively. Strain 1315 also grew by oxidation of yeast extract, glucose, xylose or lactate under a N atmosphere, reducing Fe(III) or AQDS. Yeast extract (0.2 g l) was required for growth. Isolate 1315 grew exclusively with Fe(III) or AQDS as an electron acceptor. The generation time under optimal conditions with CO as growth substrate was 9.3 h. The G+C content of the DNA was 41.5±0.5 mol%. 16S rRNA gene sequence analysis placed the organism in the genus (97.8 % similarity with the closest relative). On the basis of physiological features and phylogenetic analysis, it is proposed that strain 1315 should be assigned to a novel species, sp. nov., with the type strain 1315 (=VKPM 9905B =VKM B-2474 =DSM 21278).

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Keyword(s): AQDS, 9,10-anthraquinone 2,6-disulfonate
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2009-02-01
2024-04-19
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References

  1. Boulygina, E. S., Kuznetsov, B. B., Marusina, A. I., Tourova, T. P., Kravchenko, I. K., Bykova, S. A., Kolganova, T. V. & Galchenko, V. F.(2002). A study of nucleotide sequences of nifH genes of some methanotrophic bacteria. Microbiology (English translation of Mikrobiologiia) 71, 425–432.[CrossRef] [Google Scholar]
  2. Henstra, A. M. & Stams, J. M.(2004). Novel physiological features of Carboxydothermus and Thermoterrabacterium ferrireducens. Appl Environ Microbiol 70, 7236–7240.[CrossRef] [Google Scholar]
  3. Kevbrin, V. V. & Zavarzin, G. A.(1992). Effect of sulfur compounds on the growth of the halophilic homoacetic bacterium Acetohalobium arabaticum. Microbiology (English translation of Mikrobiologiia) 61, 563–817. [Google Scholar]
  4. Lovley, D. R., Holmes, D. E. & Nevin, K. P.(2004). Dissimilatory Fe(III) and Mn(IV) reduction. Adv Microb Physiol 49, 219–286. [Google Scholar]
  5. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  6. Slobodkin, A. I.(2005). Thermophilic microbial metal reduction. Microbiology (English translation of Mikrobiologiia) 74, 501–514.[CrossRef] [Google Scholar]
  7. Slobodkin, A. I., Reysenbach, A.-L., Strutz, N., Dreier, M. & Wiegel, J.(1997).Thermoterrabacterium ferrireducens gen. nov., sp. nov., a thermophilic anaerobic dissimilatory Fe(III)-reducing bacterium from a continental hot spring. Int J Syst Bacteriol 47, 541–547.[CrossRef] [Google Scholar]
  8. Slobodkin, A. I., Tourova, T. P., Kuznetsov, B. B., Kostrikina, N. A., Chernyh, N. A. & Bonch-Osmolovskaya, E. A.(1999).Thermoanaerobacter siderophilus sp. nov., a novel dissimilatory Fe(III)-reducing, anaerobic, thermophilic bacterium. Int J Syst Bacteriol 49, 1471–1478.[CrossRef] [Google Scholar]
  9. Slobodkin, A. I., Sokolova, T. G., Lysenko, A. M. & Wiegel, J.(2006). Reclassification of Thermoterrabacterium ferrireducens as Carboxydothermus ferrireducens comb. nov., and emended description of the genus Carboxydothermus. Int J Syst Evol Microbiol 56, 2349–2351.[CrossRef] [Google Scholar]
  10. Sokolova, T. G., Kostrikina, N. A., Chernyh, N. A., Tourova, T. P., Kolganova, T. V. & Bonch-Osmolovskaya, E. A.(2002).Carboxydocella thermautotrophica gen. nov., sp. nov., a novel anaerobic, CO-utilizing thermophile from a Kamchatkan hot spring. Int J Syst Evol Microbiol 52, 1961–1967.[CrossRef] [Google Scholar]
  11. Sokolova, T. G., Gonzales, J. M., Kostrikina, N. A., Chernyh, N. A., Slepova, T. V., Bonch-Osmolovskaya, E. A. & Robb, F. T.(2004).Thermosinus carboxydivorans gen. nov., sp. nov., a new anaerobic, thermophilic, carbon-monoxide-oxidizing, hydrogenogenic bacterium from a hot pool of Yellowstone National Park. Int J Syst Evol Microbiol 54, 2353–2359.[CrossRef] [Google Scholar]
  12. Sokolova, T. G., Kostrikina, N. A., Chernyh, N. A., Kolganova, T. V., Tourova, T. P. & Bonch-Osmolovskaya, E. A.(2005).Thermincola carboxydiphila gen. nov., sp. nov., a novel anaerobic, carboxydotrophic, hydrogenogenic bacterium from a hot spring of the Lake Baikal area. Int J Syst Evol Microbiol 55, 2069–2073.[CrossRef] [Google Scholar]
  13. Sokolova, T. G., Hanel, J., Onyenwoke, R. U., Reysenbach, A.-L., Banta, A., Geyer, R., González, J. M., Whitman, W. B. & Wiegel, J.(2007). Novel chemolithotrophic, thermophilic, anaerobic bacteria Thermolithobacter ferrireducens gen. nov., sp. nov. and Thermolithobacter carboxydivorans sp. nov. Extremophiles 11, 145–157.[CrossRef] [Google Scholar]
  14. Stackebrandt, E. & Ebers, J.(2006). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 36, 152–155. [Google Scholar]
  15. Subbotina, I. V., Chernyh, N. A., Sokolova, T. G., Kublanov, I. I., Bonch-Osmolovskaya, E. A. & Lebedinsky, A. V.(2003). Oligonucleotide probes for the detection of representatives of the genus Themoanaerobacter. Microbiology (English translation of Mikrobiologiia) 72, 331–339.[CrossRef] [Google Scholar]
  16. Svetlichny, V. A., Sokolova, T. G., Gerhardt, M., Ringpfeil, M., Kostrikina, N. A. & Zavarzin, G. A.(1991).Carboxydothermus hydrogenoformans gen. nov., sp. nov., a CO-utilizing thermophilic anaerobic bacterium from hydrothermal environments of Kunashir Island. Syst Appl Microbiol 14, 254–260.[CrossRef] [Google Scholar]
  17. Wolin, E. A., Wolin, M. J. & Wolfe, R. S.(1963). Formation of methane by bacterial extracts. J Biol Chem 238, 2882–2886. [Google Scholar]
  18. Zavarzina, D. G., Sokolova, T. G., Tourova, T. P., Chernyh, N. A., Kostrikina, N. A. & Bonch-Osmolovskaya, E. A.(2007).Thermincola ferriacetica sp. nov., a new anaerobic, thermophilic, facultatively chemolithoautotrophic bacterium capable of dissimilatory Fe(III) reduction. Extremophiles 11, 1–7.[CrossRef] [Google Scholar]
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Carboxydothermus siderophilus sp. nov., a thermophilic, hydrogenogenic, carboxydotrophic, dissimilatory Fe(III)-reducing bacterium from a Kamchatka hot spring
Int J Syst Evol Microbiol 59, 213 (2009); https://doi.org/10.1099/ijs.0.000620-0
/content/journal/ijsem/10.1099/ijs.0.000620-0
/content/journal/ijsem/10.1099/ijs.0.000620-0
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