The taxonomic position of a novel bacterial strain, YC6269, isolated from the rhizosphere of rice ( L.) managed under no-tillage practice in Jinju, South Korea, was studied using polyphasic approach. Cells of the strain were Gram-negative, rod-shaped and facultatively anaerobic. The novel strain grew at a temperature of 15–42 °C (optimum at 28 °C). Growth of the strain occurred between pH 5.5 and 11.0, with an optimum at pH 7.0–8.0. The G+C content of the total DNA was 67.4 mol%. The 16S rRNA gene sequence of the strain was most closely related to species of the genus , DSM 17635 (98.6 %), GH34-4 (97.2 %), DSM 2043 (96.9 %), DSM 17634 (96.3 %) and GH41-7 (96.2 %). The novel strain showed <96.0 % similarity with other species of the genus . Chemotaxonomic data (major quinone, Q-8; major polar lipids, phosphatidylethanolamine, phosphatidylglycerol and phosphatidyl--methylethanolamine, and major fatty acids, C iso, C iso, C iso and C iso 9) supported the affiliation of strain YC6269 to the genus . Phylogenetic analysis based on the 16S rRNA gene sequences, DNA–DNA hybridization data and biochemical and physiological characteristics strongly supported the genotypic and phenotypic differentiation of strain YC6269 from recognized species of the genus . Strain YC6269, therefore, represents a novel member of the genus , for which the name sp. nov. is proposed. The type strain is YC6269 (=KCTC 22249=DSM 21044).


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