Strain 78-123T was isolated from a sample of a bird's nest situated on the bank of Qiongtailan River in the region of Tuomuer Peak of Tianshan Mountain in the Xin-jiang Uygur Autonomous Region in north-western China. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 78-123T was related to members of the genus Pseudomonas. 16S rRNA gene sequence similarity between strain 78-123T and Pseudomonas mendocina ATCC 25411T, Pseudomonas pseudoalcaligenes JCM 5968T and Pseudomonas alcaliphila AL15-21T was 97.1, 97.4 and 97.5 %, respectively. The major cellular fatty acids were C16 : 0, C16 : 1ω7c and/or iso-C15 : 0 2-OH, C18 : 1ω7c and C12 : 0. The G+C content was 60.4 mol%. On the basis of the phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, the novel species Pseudomonas tuomuerensis sp. nov. is proposed, with the type strain 78-123T (=CGMCC 1.1365T =JCM 14085T).
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[PDF file of Supplementary
Table S1 and Supplementary Fig. S1](100 KB)
Supplementary Fig. S2. Transmission electron micrographs
of cells of strain 78-123
Tshowing a cell with polar multitrichous flagella
(top) and a cell which has become twisted into a fascicle or
bundle (bottom). Bars, 1 µm (top) and 0.5 µm
(bottom).