1887

Abstract

Strain 78-123 was isolated from a sample of a bird's nest situated on the bank of Qiongtailan River in the region of Tuomuer Peak of Tianshan Mountain in the Xin-jiang Uygur Autonomous Region in north-western China. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 78-123 was related to members of the genus . 16S rRNA gene sequence similarity between strain 78-123 and ATCC 25411, JCM 5968 and AL15-21 was 97.1, 97.4 and 97.5 %, respectively. The major cellular fatty acids were C, C 7 and/or iso-C 2-OH, C 7 and C. The G+C content was 60.4 mol%. On the basis of the phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, the novel species sp. nov. is proposed, with the type strain 78-123 (=CGMCC 1.1365 =JCM 14085).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.000547-0
2009-01-01
2020-01-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/1/139.html?itemId=/content/journal/ijsem/10.1099/ijs.0.000547-0&mimeType=html&fmt=ahah

References

  1. Anzai, Y., Kim, H., Park, J.-Y., Wakabayashi, H. & Oyaizu, H. ( 2000; ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. Int J Syst Evol Microbiol 50, 1563–1589.[CrossRef]
    [Google Scholar]
  2. Collins, M. D. ( 1985; ). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  3. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  4. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  5. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  6. Hauser, E., Kämpfer, P. & Busse, H.-J. ( 2004; ). Pseudomonas psychrotolerans sp. nov. Int J Syst Evol Microbiol 54, 1633–1637.[CrossRef]
    [Google Scholar]
  7. Hu, H. Y., Lim, B. R., Goto, N. & Fujie, K. ( 2001; ). Analytical precision and repeatability of respiratory quinones for quantitative study of microbial community structure in environmental samples. J Microbiol Methods 47, 17–24.[CrossRef]
    [Google Scholar]
  8. Hugh, R. & Leifson, E. ( 1953; ). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66, 24–26.
    [Google Scholar]
  9. Kates, M. ( 1986; ). Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier.
  10. King, E. O., Ward, M. K. & Raney, D. E. ( 1954; ). Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med 44, 301–307.
    [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega 3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  12. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. London: Wiley.
  13. Las Heras, A., Domínguez, L., López, I. & Fernández-Garayzábal, J. F. ( 1999; ). Outbreak of acute ovine mastitis associated with Pseudomonas aeruginosa infection. Vet Rec 145, 111–112.[CrossRef]
    [Google Scholar]
  14. Marmur, J. ( 1961; ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef]
    [Google Scholar]
  15. Marmur, J. & Doty, P. ( 1962; ). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef]
    [Google Scholar]
  16. Palleroni, N. J. ( 1984; ). Genus I. Pseudomonas Migula 1894, 237AL (nom. cons. Opin. 5, Jud. Comm. 1952, 237). In Bergey's Manual of Systematic Bacteriology, vol. 1, pp. 141–199. Edited by N. R. Krieg & J. G. Holt. Baltimore: Williams & Wilkins.
  17. Palleroni, N. J. ( 2005; ). Genus I. Pseudomonas Migula 1894, 237AL (nom. cons. Opin. 5 of the Jud. Comm. 1952, 121). In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2, part B, pp. 323–379. Edited by D. J. Brenner, N. R. Krieg, J. T. Staley & G. M. Garrity. New York: Springer.
  18. Palleroni, N. J., Kunisawa, R., Contopoulou, R. & Doudoroff, M. ( 1973; ). Nucleic acid homologies in the genus Pseudomonas. Int J Syst Bacteriol 23, 333–339.[CrossRef]
    [Google Scholar]
  19. Prakash, O., Kumari, K. & Lal, R. ( 2007; ). Pseudomonas delhiensis sp. nov., from a fly ash dumping site of a thermal power plant. Int J Syst Evol Microbiol 57, 527–531.[CrossRef]
    [Google Scholar]
  20. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  21. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids. Technical Note 101. Newark, DE: MIDI.
  22. Smibert, R. M. & Krieg, N. R. ( 1981; ). General characterization. In Manual of Methods for General Bacteriology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  23. Stanier, R. Y., Palleroni, N. J. & Doudoroff, M. ( 1966; ). The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43, 159–271.[CrossRef]
    [Google Scholar]
  24. Stolz, A., Busse, H.-J. & Kämpfer, P. ( 2007; ). Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 57, 572–576.[CrossRef]
    [Google Scholar]
  25. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
  26. Tvrzová, L., Schumann, P., Spröer, C., Sedláček, I., Páčová, Z., Šedo, O., Zdráhal, Z., Steffen, M. & Lang, E. ( 2006; ). Pseudomonas moraviensis sp. nov. and Pseudomonas vranovensis sp. nov., soil bacteria isolated on nitroaromatic compounds, and emended description of Pseudomonas asplenii. Int J Syst Evol Microbiol 56, 2657–2663.[CrossRef]
    [Google Scholar]
  27. Wu, C., Lu, X., Qin, M., Wang, Y. & Ruan, J. ( 1989; ). Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 16, 176–178.
    [Google Scholar]
  28. Yumoto, I., Yamazaki, K., Hishinuma, M., Nodasaka, Y., Suemori, A., Nakajima, K., Inoue, N. & Kawasaki, K. ( 2001; ). Pseudomonas alcaliphila sp. nov., a novel facultatively psychrophilic alkaliphile isolated from seawater. Int J Syst Evol Microbiol 51, 349–355.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.000547-0
Loading
/content/journal/ijsem/10.1099/ijs.0.000547-0
Loading

Data & Media loading...

Supplements

[PDF file of Supplementary Table S1 and Supplementary Fig. S1](100 KB)

PDF

Transmission electron micrographs of cells of strain 78-123 showing a cell with polar multitrichous flagella (top) and a cell which has become twisted into a fascicle or bundle (bottom). Bars, 1 µm (top) and 0.5 µm (bottom).

IMAGE



IMAGE

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error