A Gram-negative, non-spore-forming, rod-shaped bacterium, designated strain E3, which was motile with a polar flagellum, was isolated from coastal surface seawater near Tianjin city, China. The taxonomy of strain E3 was studied by phenotypic, chemotaxonomic and phylogenetic methods. Strain E3 forms non-pigmented colonies with a butyrous–mucoid consistency on marine 2216 agar. It grows optimally at 30 °C, in the presence of 2.0 % (w/v) NaCl and at pH 7.6. Strain E3 is positive for catalase and oxidase and hydrolysis of lipid and reduces nitrate to N gas. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain E3 belongs to the and is grouped in the genus , with highest sequence similarity to the type strains of (94.6 %), (94.4 %) and (94.4 %). Strain E3 contained summed feature 3 (C 7 and/or iso-C 2-OH, 34.5 %), C 7 (17.3 %) and C (17 %) as the major fatty acids and phosphatidylethanolamine and phosphatidylglycerol as the main phospholipids. The DNA G+C content was 40.8 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain E3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is E3 (=CGMCC 1.7001 =JCM 15139).


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Transmission electron micrographs of cells of strain E3 after negative staining with 1 % (w/v) phosphotungstic acid, showing flagella (F), prosthecae (P) and buds (B). Cells were grown on MA at 30 °C for 2 days. Bars, 500 nm.


Polar lipid profile of strain E3 . Polar lipids were examined by two-dimensional TLC and characterized with spraying reagents specific for α-glycols (periodate–Schiff), sugars (α-naphthol/sulfuric acid, anisaldehyde/sulfuric acid), free amino groups (ninhydrin) and phosphate (Zindzadze). A 50 % sulfuric acid solution was used to detect spots of all kinds of lipids. -P, -phosphatidic acid; PE, phosphatidylethanolamine; PG, phosphatidylglycerol; GL1, unknown glycolipid.


Comparison of cellular fatty acid profile of strain E3 with those of type strains of species. [PDF](70 KB)


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