Three halophilic isolates, strains Halo-G*, AUS-1 and Naxos II, were compared. Halo-G* was isolated from an evaporitic salt crystal from Baja California, Mexico, whereas AUS-1 and Naxos II were isolated from salt pools in Western Australia and the Greek island of Naxos, respectively. Halo-G* had been exposed previously to conditions of outer space and survived 2 weeks on the Biopan facility. Chemotaxonomic and molecular comparisons suggested high similarity between the three strains. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the strains clustered with species, showing sequence similarities of 99.2–97.1 %. The DNA–DNA hybridization values of strain Halo-G* and strains AUS-1 and Naxos II are 73 and 75 %, respectively, indicating that they constitute a single species. The DNA relatedness between strain Halo-G* and the type strains of 13 closely related species of the genus ranged from 39 to 2 %, suggesting that the three isolates constitute a different genospecies. The G+C content of the DNA of the three strains was 65.5–66.5 mol%. All three strains contained CC derivatives of diethers of phosphatidylglycerol, phosphatidylglyceromethylphosphate and phosphatidylglycerolsulfate, together with a sulfated glycolipid. On the basis of these results, a novel species that includes the three strains is proposed, with the name sp. nov. The type strain is strain Halo-G* (=DSM 19316 =NCIMB 14426 =ATCC BAA-1602).


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vol. , part 8, pp. 1908 - 1913

Phase-contrast micrographs of cells of strains Halo-G* and Naxos II from liquid cultures grown under optimal conditions to late-exponential phase.

Two-dimensional TLC of polar lipids extracted from strains Halo-G* , Naxos II and AUS-1.

[PDF file of Supplementary Figs S1 and S2](96 KB)

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